Raman spectroscopy for monitoring free sulfhydryl formation during monoclonal antibody manufacturing

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DOI:10.1016/j.jpba.2024.116530
作者：Zhenshu Wang , Andrew Hsieh , Patricia Rose , George Zhou , Sonja Battle , Kelly Raymond , Monica Haley , Aaron Cote , Sandra Bennun , Sanjeev Ahuja
文献类型：期刊论文
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Elsevier

Abstract：During production, harvested cell culture fluid (HCCF) can degrade due to reductases breaking interchain disulfide bonds, forming low molecular weight (LMW) impurities that contain free sulf

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