A Minimal and Portable CRISPR Platform Based on Bifidobacterial Cas9 Enables Genome Editing in E. coli Nissle 1917.

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DOI:10.1021/acssynbio.5c00911
作者：Qibing Liu, Yan Huang, Ran Zhou, Honghao Ding, Qingqing Nie, Xiaoyuan Gong, Ting Zuo, Shuai Wang, Yunxiang Liang, Yingjun Li
文献类型：期刊论文
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American Chemical Society (ACS)

Abstract：Genetic manipulation of core gut probiotics remains challenging due to endogenous cellular barriers and a scarcity of efficient molecular tools, limiting progress in live biotherapeutic deve

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