病毒载量和宿主细胞因子在确定儿童呼吸道合胞病毒相关急性下呼吸道感染疾病严重程度中的作用

IF 1.3 4区 医学 Q4 INFECTIOUS DISEASES
Subhabrata Sarkar, Radha Kanta Ratho, Meenu Singh, Mini Pritam Singh, Amarjeet Singh, Megha Sharma
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引用次数: 0

摘要

呼吸道合胞病毒(RSV)是急性下呼吸道感染(ALRTIs)的主要原因。在这项研究中,我们旨在评估病毒载量、细胞因子、基质金属蛋白酶9 (MMP-9)和金属蛋白酶1组织抑制剂(TIMP-1)在确定RSV疾病严重程度中的作用,并确定疾病严重程度的潜在生物标志物。在2013年12月至2016年3月期间出现呼吸道合胞病毒感染的142例患者(年龄在2个月至5岁之间)被纳入研究。对患者的鼻咽分泌物进行RSV病毒载量定量检测,并用细胞因子头阵列检测局部细胞因子IL-6 (IL-6)、肿瘤坏死因子α (TNF-α)、IL-17A、干扰素γ (IFN-γ)和IL-10水平。采用定量酶联免疫吸附试验(Quantikine ELISA)计算109例抽吸者的MMP-9和TIMP-1水平。对不同疾病严重程度类别的这些参数进行比较。较高的病毒载量和升高的TNF-α、MMP-9和MMP-9:TIMP-1水平与更严重的疾病相关;而IL-17A、IFN-γ和IFN-γ:IL-10的水平与疾病消退有关。在定义从非严重疾病到严重疾病的转变时,MMP-9的敏感性和特异性分别为89.7%和85.4%。MMP-9:TIMP-1的敏感性和特异性分别为87.2%和76.8%。因此,MMP-9、MMP-9:TIMP-1、TNF-α和IL-10可以作为rsv感染儿童疾病进展的潜在生物标志物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Role of Viral Load and Host Cytokines in Determining the Disease Severity of Respiratory Syncytial Virus-Associated Acute Lower Respiratory Tract Infections in Children.

Respiratory syncytial virus (RSV) is a major cause of acute lower respiratory tract infections (ALRTIs). In this study, we aimed to evaluate the role of viral load, cytokines, matrix metalloproteinase 9 (MMP-9), and tissue inhibitor of metalloproteinase 1 (TIMP-1) in determining the severity of RSV disease and identify potential biomarkers of disease severity. A total of 142 patients with RSV infection (aged between 2 months and 5 years) who presented with ALRTI between December 2013 and March 2016 were enrolled. Their nasopharyngeal aspirates were subjected to RSV viral load quantification, and local cytokine levels of interleukin 6 (IL-6), tumor necrosis factor α (TNF-α), IL-17A, interferon γ (IFN-γ), and IL-10 were determined using a cytokine bead array. The levels of MMP-9 and TIMP-1 in 109 aspirates were calculated using Quantikine ELISA. These parameters were compared for different disease severity categories. A higher viral load and increased levels of TNF-α, MMP-9, and MMP-9:TIMP-1 were associated with greater severity of disease; whereas levels of IL-17A, IFN-γ, and IFN-γ:IL-10 were associated with disease resolution. When defining the transition from non-severe to severe disease, MMP-9 had a sensitivity and specificity of 89.7% and 85.4%, respectively. Moreover, MMP-9:TIMP-1 had a sensitivity and specificity of 87.2% and 76.8%, respectively. Hence, MMP-9, MMP-9:TIMP-1, TNF-α, and IL-10 could serve as potential biomarkers for disease progression in RSV-infected children.

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来源期刊
CiteScore
4.50
自引率
4.50%
发文量
172
审稿时长
2 months
期刊介绍: Japanese Journal of Infectious Diseases (JJID), an official bimonthly publication of National Institute of Infectious Diseases, Japan, publishes papers dealing with basic research on infectious diseases relevant to humans in the fields of bacteriology, virology, mycology, parasitology, medical entomology, vaccinology, and toxinology. Pathology, immunology, biochemistry, and blood safety related to microbial pathogens are among the fields covered. Sections include: original papers, short communications, epidemiological reports, methods, laboratory and epidemiology communications, letters to the editor, and reviews.
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