多种方法的综合分析揭示了髓母细胞瘤免疫微环境的特征。

IF 2.7 3区 医学 Q2 CLINICAL NEUROLOGY
Brain Tumor Pathology Pub Date : 2023-10-01 Epub Date: 2023-08-09 DOI:10.1007/s10014-023-00467-4
Kaiyu Fan, Yifan Wei, Yunwei Ou, Jian Gong
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引用次数: 0

摘要

为了通过流式细胞术(FCM)、免疫组化(IHC)、大量RNA表达和单细胞RNA测序(scRNA-seq)四种方法探讨髓母细胞瘤(MB)免疫微环境(IME)的特征,我们收集了MB、室管膜瘤(EPN)、高级别胶质瘤(HGG)和低级别胶质瘤的术中标本,进行了跨癌比较。分别对标本进行FCM和IHC,并在GEO数据库的MB中对大块RNA表达数据进行反褶积和scRNA-seq分析。FCM和IHC分析发现MB与其他脑肿瘤的淋巴细胞(LC)和T细胞比例存在显著差异。对大量RNA表达数据的反褶积显示,MCPCOUNTER中只有细胞类型的比例发生了很大变化。scRNA-seq发现,不同亚型的MB IME中各种免疫细胞的比例不同。FCM、IHC、批量RNA表达和scRNA-seq等技术可以在一定程度上分类不同的免疫细胞亚群并量化其比例。这四种方法都有各自的优势和局限性,但对于MB等高度异质性肿瘤,多种方法的综合分析是更好的选择。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Integrated analysis of multiple methods reveals characteristics of the immune microenvironment in medulloblastoma.

Integrated analysis of multiple methods reveals characteristics of the immune microenvironment in medulloblastoma.

To explore the characteristics of the immune microenvironment (IME) of medulloblastoma (MB) by four methods: flow cytometry (FCM), immunohistochemical (IHC), bulk RNA expression and single cell RNA sequencing (scRNA-seq), we collected the intraoperative specimens of MB, ependymoma (EPN), high-grade glioma (HGG), and low-grade glioma (LGG) to make a cross-cancer comparison. The specimens were subjected to FCM and IHC respectively, and deconvolution from bulk RNA expression data and scRNA-seq analysis were performed in MB from the GEO database. FCM and IHC analysis found that the proportion of lymphocytes (LC) and T cells between MB and other brain tumors were significantly different. The deconvolution of bulk RNA expression data showed that only the proportion of cell types in MCPCOUNTER changed greatly. scRNA-seq found that the proportion of various immune cells in the IME of MB differed between different subtypes. Techniques such as FCM, IHC, bulk RNA expression, and scRNA-seq can sort out different immune cell subsets to a certain extent and quantify their proportions. The four methods have their own strengthens and limitations, but for highly heterogeneous tumor such as MB, integrated analysis of multiple methods is a better choice.

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来源期刊
Brain Tumor Pathology
Brain Tumor Pathology 医学-病理学
CiteScore
5.40
自引率
9.10%
发文量
30
审稿时长
>12 weeks
期刊介绍: Brain Tumor Pathology is the official journal of the Japan Society of Brain Tumor Pathology. This international journal documents the latest research and topical debate in all clinical and experimental fields relating to brain tumors, especially brain tumor pathology. The journal has been published since 1983 and has been recognized worldwide as a unique journal of high quality. The journal welcomes the submission of manuscripts from any country. Membership in the society is not a prerequisite for submission. The journal publishes original articles, case reports, rapid short communications, instructional lectures, review articles, letters to the editor, and topics.Review articles and Topics may be recommended at the annual meeting of the Japan Society of Brain Tumor Pathology. All contributions should be aimed at promoting international scientific collaboration.
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