泼尼松龙能改善三甲基锡诱导的神经退行性病变后的海马再生,并能防止T淋巴细胞浸润。

IF 1.3 4区 医学 Q4 CLINICAL NEUROLOGY
Neuropathology Pub Date : 2024-02-01 Epub Date: 2023-06-08 DOI:10.1111/neup.12926
Masashi Sakurai, Miki Takenaka, Yuki Mitsui, Yusuke Sakai, Masahiro Morimoto
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引用次数: 0

摘要

大脑的内源性再生能力很弱,但有报道称,在大脑病变部位会出现再生反应,即产生新的神经元(神经发生)。此外,众所周知,白细胞会浸润脑损伤部位。因此,白细胞也与再生神经元的产生有关,但其作用尚未完全阐明。在本研究中,我们在注射三甲基锡(TMT)的小鼠海马再生模型中研究了白细胞浸润及其对脑组织再生的影响。免疫组化结果显示,在注射三甲基锡的小鼠海马病灶中发现了 CD3 阳性的 T 淋巴细胞。泼尼松龙(PSL)治疗抑制了T淋巴细胞的浸润,并增加了海马中神经元核(NeuN)阳性的成熟神经元和双皮质素(DCX)阳性的未成熟神经元。对溴脱氧尿苷(BrdU)标记的新生细胞进行的调查显示,PSL 处理后,BrdU/NeuN 和 BrdU/DCX 阳性细胞的百分比有所增加。这些结果表明,浸润的 T 淋巴细胞通过抑制海马神经发生来阻止脑组织再生。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Prednisolone improves hippocampal regeneration after trimethyltin-induced neurodegeneration in association with prevention of T lymphocyte infiltration.

The endogenous regenerative capacity of the brain is quite weak; however, a regenerative reaction, the production of new neurons (neurogenesis), has been reported to occur in brain lesions. In addition, leukocytes are well known to infiltrate brain lesions. Therefore, leukocytes would also have a link with regenerative neurogenesis; however, their role has not been fully elucidated. In this study, we investigated leukocyte infiltration and its influence on brain tissue regeneration in a trimethyltin (TMT)-injected mouse model of hippocampal regeneration. Immunohistochemically, CD3-positive T lymphocytes were found in the hippocampal lesion of TMT-injected mice. Prednisolone (PSL) treatment inhibited T lymphocyte infiltration and increased neuronal nuclei (NeuN)-positive mature neurons and doublecortin (DCX)-positive immature neurons in the hippocampus. Investigation of bromodeoxyuridine (BrdU)-labeled newborn cells revealed the percentage of BrdU/NeuN- and BrdU/DCX-positive cells increased by PSL treatment. These results indicate that infiltrated T lymphocytes prevent brain tissue regeneration by inhibiting hippocampal neurogenesis.

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来源期刊
Neuropathology
Neuropathology 医学-病理学
CiteScore
4.10
自引率
4.30%
发文量
105
审稿时长
6-12 weeks
期刊介绍: Neuropathology is an international journal sponsored by the Japanese Society of Neuropathology and publishes peer-reviewed original papers dealing with all aspects of human and experimental neuropathology and related fields of research. The Journal aims to promote the international exchange of results and encourages authors from all countries to submit papers in the following categories: Original Articles, Case Reports, Short Communications, Occasional Reviews, Editorials and Letters to the Editor. All articles are peer-reviewed by at least two researchers expert in the field of the submitted paper.
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