精浆ExLncRNA对:WGCNA在mTESE非阻塞性无精子症患者中寻找睾丸精子检索生物标志物的最新视角

IF 1.5 4区医学 Q3 UROLOGY & NEPHROLOGY

Urology Journal Pub Date : 2023-07-26 DOI:10.22037/uj.v20i.7323

Haiming Cao, Chuntao Wang, Ruilin Cai, Zi Wan, Lin Ma

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引用次数: 0

摘要

目的:本研究旨在从精浆exLncRNA对中寻找睾丸取回精子的候选生物标志物。材料和方法:选择一组具有最佳潜在生物标志物的exLncRNA对，并在96份NOA样本中进行验证。加权相关网络分析(WGCNA)和最小绝对收缩和选择算子(LASSO)用于确定这些配对的可能生物标志物。这些配对的潜在生物标记物使用接受者操作曲线进行鉴定。计算混淆矩阵和敏感性、特异性、阳性预测值(PPV)、阴性预测值(NPV)、FP、假阴性率(FNR)和F1评分。通过F1得分，我们选择了最佳的阈值。结果:验证了各对在睾丸取精(+)和睾丸取精(-)男性中的相对差异表达。这6对组合显示出最好的生物标记潜力。其中，CCDC37。在选定和验证的队列中，DT-LOCI00505685和LOC440934- LOCI01929088 (XR_001745218.1)对检测睾丸精子检索显示出最显著的潜力和稳定性。结论:CCDC37。DT-LOCI00505685对和LOC440934- LOCI01929088 (XR_001745218.1)对有潜力成为新的分子生物标志物，可以帮助临床选择显微解剖睾丸精子提取策略。

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Seminal Plasma ExLncRNA Pairs: Updating Perspectives in the Search for Testicular Spermatozoa Retrieval Biomarkers in Nonobstructive Azoospermia Patients with mTESE by WGCNA.

Purpose: This study aims to find candidates for testicular spermatozoa retrieval biomarkers among the seminal plasma exLncRNA pairs.

Materials and methods: A set of exLncRNA pairs with the best potential biomarkers was selected and validated in 96 NOA samples. Weighted correlation network analysis (WGCNA) and Least Absolute Shrinkage and Selection Operator were used to identify possible biomarkers for these pairs (LASSO). These pairs' potential biomarkers were identified using receiver operating curves. Confusion matrices and sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), FP, false-negative rates (FNR), and F1 scores are calculated. Through F1 scores, we selected the best threshold value.

Results: The relative differential expression of each pair in testicular spermatozoa retrieval (+) and testicular spermatozoa retrieval (-) men were validated. The six pairs displayed the best biomarker potential. Among them, CCDC37.DT-LOCI00505685 pair and LOC440934- LOCI01929088 (XR_001745218.1) pair showed the most significant potential and stability for detecting testicular spermatozoa retrieval in the selected and validated cohort.

Conclusion: CCDC37.DT-LOCI00505685 pair and LOC440934- LOCI01929088 (XR_001745218.1) pair have the potential to become new molecular biomarkers that could help to select clinical strategies for microdissection testicular sperm extraction.

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来源期刊

Urology Journal UROLOGY & NEPHROLOGY-

CiteScore

2.60

自引率

6.70%

发文量

审稿时长

6-12 weeks

期刊介绍： As the official journal of the Urology and Nephrology Research Center (UNRC) and the Iranian Urological Association (IUA), Urology Journal is a comprehensive digest of useful information on modern urology. Emphasis is on practical information that reflects the latest diagnostic and treatment techniques. Our objectives are to provide an exceptional source of current and clinically relevant research in the discipline of urology, to reflect the scientific work and progress of our colleagues, and to present the articles in a logical, timely, and concise format that meets the diverse needs of today’s urologist. Urology Journal publishes manuscripts on urology and kidney transplantation, all of which undergo extensive peer review by recognized authorities in the field prior to their acceptance for publication. Accordingly, original articles, case reports, and letters to editor are encouraged.