细胞骨架调节RNA和细胞周期蛋白依赖性激酶抑制剂2B基因在癌症中的表达分析。

Q3 Medicine

Human Antibodies Pub Date : 2023-01-01 DOI:10.3233/HAB-220015

Majid Mokhtari, Mahdi Gholipour, Solat Eslami, Atefe Abak, Bashdar Mahmud Hussen, Azadeh Rakhshan, Soudeh Ghafouri-Fard

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引用次数: 0

摘要

背景：癌症已被发现与几种非编码基因和mRNA编码基因的失调有关。目的：探讨乳腺癌症及癌旁组织中CYTOR和CDKN2B的表达及其与临床资料的相关性。方法：采用实时PCR方法检测43例新诊断的乳腺癌症及其癌旁标本中CDKN2B和CYTOR的表达水平。结果：肿瘤中的细胞因子水平高于邻近组织。然而，CDKN2B在这两组组织之间的表达没有差异。ROC曲线分析显示CYTOR水平可以区分肿瘤和邻近组织，细胞因子和CDKN2B基因在乳腺癌症组织及癌旁组织中的表达水平呈正相关（r＝0.5，P＝0.0008），CDKN2B在正常组织中的相对表达水平与临床分期有关（P＝0.014），CDKN2B在肿瘤组织中的相对表达水平与体重有关。CYTOR和CDKN2B的表达与临床或病理变量之间没有其他关联。结论：本研究为这些基因参与癌症的病理病因提供了证据。

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Expression analysis of cytoskeleton regulator RNA and Cyclin Dependent Kinase Inhibitor 2B genes in breast cancer.

Background: Breast cancer has been found to be associated with deregulation of several non-coding genes and mRNA coding genes.

Objective: To assess expressions of CYTOR and CDKN2B in breast cancer and adjacent samples and find their relevance with clinical data.

Methods: We enumerated expression level of CDKN2B and CYTOR in 43 newly diagnosed breast cancer samples and their adjacent specimens using real-time PCR method Expression data was judged using Wilcoxon matched-pairs signed rank test.

Results: CYTOR level was higher in tumors compared with adjacent tissues. Nevertheless, there was no difference in expression of CDKN2B between these two sets of tissues. ROC curve analysis showed that CYTOR levels can differentiate between tumoral and adjacent tissues with AUC, specificity and sensitivity values of 0.65, 37% and 92% (P= 0.017). There was a positive correlation between expression levels of CYTOR and CDKN2B genes in breast cancer tissues (r= 0.5 and P= 0.0008) as well as adjacent tissues (r= 0.79 and P< 0.0001). Relative expression level of CDKN2B in normal tissues was associated with clinical stage (P= 0.014). Moreover, relative expression level of CDKN2B in tumor tissues was associated with the body weight. There was no other association between expressions of CYTOR and CDKN2B and clinical or pathological variables.

Conclusions: Cumulatively, this study offers evidence for involvement of these genes in the pathoetiology of breast cancer.

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来源期刊

Human Antibodies Medicine-Immunology and Allergy

CiteScore

3.50

自引率

0.00%

发文量

期刊介绍： Human Antibodies is an international journal designed to bring together all aspects of human hybridomas and antibody technology under a single, cohesive theme. This includes fundamental research, applied science and clinical applications. Emphasis in the published articles is on antisera, monoclonal antibodies, fusion partners, EBV transformation, transfections, in vitro immunization, defined antigens, tissue reactivity, scale-up production, chimeric antibodies, autoimmunity, natural antibodies/immune response, anti-idiotypes, and hybridomas secreting interesting growth factors. Immunoregulatory molecules, including T cell hybridomas, will also be featured.