利用亲水作用液相色谱-质谱法同时测定水溶性除草剂。

IF 2.8 4区 医学 Q2 TOXICOLOGY
Forensic Toxicology Pub Date : 2024-01-01 Epub Date: 2023-07-22 DOI:10.1007/s11419-023-00669-7
Daisuke Watanabe, Shuhei Sonoda, Hikoto Ohta
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引用次数: 0

摘要

目的:分析水溶性除草剂,包括草甘膦(Glyp)、草铵膦(Gluf)、百草枯(PQ)和敌草快(DQ),既费时又费钱,因为它们不能用一般的毒理学筛选方法进行分析。因此,本研究旨在开发一种简单快速的方法,在不使用任何衍生或离子配对试剂的情况下同时分析这些化合物:方法:使用亲水相互作用液相色谱法分离分析物,并使用串联质谱法进行检测。结果:所有分析物均能很好地分离并检测到:结果:所有分析物均分离良好,检测峰形良好。在植物样品中,采用简单的提取方法就能从枯叶中检测到除草剂。对生物样品进行了定量分析,Glyp 和 Gluf 的定量限为 0.2 µg/mL,PQ 和 DQ 的定量限为 1 ng/mL:结论:所开发的方法具有良好的性能,可用于实际的法医应用,包括中毒案件和恶意使用农药破坏经济作物的案件。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Simultaneous determination of water-soluble herbicides using hydrophilic interaction liquid chromatography-mass spectrometry.

Simultaneous determination of water-soluble herbicides using hydrophilic interaction liquid chromatography-mass spectrometry.

Purpose: The analysis of water-soluble herbicides, including glyphosate (Glyp), glufosinate (Gluf), paraquat (PQ), and diquat (DQ), is time-consuming and expensive because they cannot be analyzed using general toxicological screening methods. Thus, this study aimed to develop a simple and rapid method to simultaneously analyze these compounds without any derivatization nor ion-pairing reagents.

Methods: The analytes were separated using hydrophilic interaction liquid chromatography and detected using tandem mass spectrometry. The developed method was applied to plant and biological samples assuming criminal damage and poisoning cases, respectively.

Results: All analytes were separated well and detected with good peak shapes. For plant samples, the herbicides were specifically detected from withered leaves using a simple extraction method. For biological samples, quantitative analysis was successfully validated, and the limit of quantification values of Glyp and Gluf were 0.2 µg/mL, and those of PQ and DQ were 1 ng/mL.

Conclusion: The developed method had sufficient performance for practical forensic applications including poisoning cases and malicious uses to damage commercial crops.

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来源期刊
Forensic Toxicology
Forensic Toxicology TOXICOLOGY-
CiteScore
5.80
自引率
9.10%
发文量
40
审稿时长
3 months
期刊介绍: The journal Forensic Toxicology provides an international forum for publication of studies on toxic substances, drugs of abuse, doping agents, chemical warfare agents, and their metabolisms and analyses, which are related to laws and ethics. It includes original articles, reviews, mini-reviews, short communications, and case reports. Although a major focus of the journal is on the development or improvement of analytical methods for the above-mentioned chemicals in human matrices, appropriate studies with animal experiments are also published. Forensic Toxicology is the official publication of the Japanese Association of Forensic Toxicology (JAFT) and is the continuation of the Japanese Journal of Forensic Toxicology (ISSN 0915-9606).
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