基质细胞衍生因子1(SDF-1)在离体和体外测定中增加了毛细血管细胞的数量。

IF 2.1 4区 生物学 Q4 CELL BIOLOGY
Histochemistry and Cell Biology Pub Date : 2023-11-01 Epub Date: 2023-07-20 DOI:10.1007/s00418-023-02223-3
Bruno Domingos Azevedo Sanches, Guilherme Henrique Tamarindo, Alana Della Torre da Silva, Gustavo Matheus Amaro, Juliana Dos Santos Maldarine, Vitória Alário Dos Santos, Luiz Henrique Alves Guerra, Carolina Marques Bedolo Baraldi, Rejane Maira Góes, Sebastião Roberto Taboga, Hernandes F Carvalho
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引用次数: 0

摘要

Telocytes是存在于各种组织中的间质细胞,具有称为telopodes的长细胞质突起,并被归类为CD34+细胞。Telopodes形成广泛的网络,渗透到基质中,有证据表明这些网络连接了几种基质细胞类型,使它们在细胞间通讯和组织组织组织维持中发挥重要作用。数据还表明,这些网络可能受损,并且与许多病理状况(如癌症和纤维化)相关的毛细血管细胞数量减少。因此,促进端粒细胞增殖的技术已成为一个重要的治疗靶点。在本研究中,进行了离体和体外测定,以评估SDF-1对前列腺毛细血管细胞的影响,SDF-1是一种参与CD34+细胞增殖和迁移的因子。SDF-1导致外植体中端粒细胞数量的增加,以及可能与这些细胞增殖有关的形态学变化。这些变化涉及末端足类节段的融合,与细胞体体积的增加有关。体外测定还显示SDF-1富集了具有端粒细胞的前列腺基质细胞。总之,数据表明SDF-1可能在旨在增加毛细血管细胞数量的治疗中提供有前景的用途。然而,还需要进一步的研究来证实该因子在不同组织/病理条件下的有效性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Stromal cell-derived factor 1 (SDF-1) increases the number of telocytes in ex vivo and in vitro assays.

Stromal cell-derived factor 1 (SDF-1) increases the number of telocytes in ex vivo and in vitro assays.

Telocytes are interstitial cells that are present in various tissues, have long cytoplasmic projections known as telopodes, and are classified as CD34+ cells. Telopodes form extensive networks that permeate the stroma, and there is evidence that these networks connect several stromal cell types, giving them an important role in intercellular communication and the maintenance of tissue organisation. Data have also shown that these networks can be impaired and the number of telocytes reduced in association with many pathological conditions such as cancer and fibrosis. Thus, techniques that promote telocyte proliferation have become an important therapeutic target. In this study, ex vivo and in vitro assays were conducted to evaluate the impact on prostatic telocytes of SDF-1, a factor involved in the proliferation and migration of CD34+ cells. SDF-1 caused an increase in the number of telocytes in explants, as well as morphological changes that were possibly related to the proliferation of these cells. These changes involved the fusion of telopode segments, linked to an increase in cell body volume. In vitro assays also showed that SDF-1 enriched prostate stromal cells with telocytes. Altogether, the data indicate that SDF-1 may offer promising uses in therapies that aim to increase the number of telocytes. However, further studies are needed to confirm the efficiency of this factor in different tissues/pathological conditions.

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来源期刊
Histochemistry and Cell Biology
Histochemistry and Cell Biology 生物-细胞生物学
CiteScore
4.90
自引率
8.70%
发文量
112
审稿时长
1 months
期刊介绍: Histochemistry and Cell Biology is devoted to the field of molecular histology and cell biology, publishing original articles dealing with the localization and identification of molecular components, metabolic activities and cell biological aspects of cells and tissues. Coverage extends to the development, application, and/or evaluation of methods and probes that can be used in the entire area of histochemistry and cell biology.
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