优化菌血症检测。综合使用 FilmArray (BCID2) 和 MALDI-TOF。

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-10-01 DOI:10.1016/j.ram.2023.06.001

Natalia A. Carrion , Rodolfo E. Quiroz , Camila Asenzo , Manuela Gamarra , Antonio E. Flores , Maria Garcia , Rolando N. Soloaga , Julia Grigioni , Maria Mastroianni , Alejandra Margari , Facundo Molina , Adriana N. Procopio , Vanesa Reijtman , Maria S. Ratti , Victor Mamani , Myriam Vazquez , Diana Viale , Guadalupe Perez

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引用次数: 0

摘要

分析了从培养 3-5 小时的亚培养物中联合使用 MALDI-TOF MS 和 BCID2 面板（FilmArray）鉴定阳性血液培养物中微生物的实用性及其在调整抗菌治疗中的重要性。BCID2 的总体鉴定率为 90.4%（142/157），Maldi-TOF MS 的鉴定率为 83.4%（131/157）（p = 0.0858）；在 23 个多微生物病例（47 株）中，BCID2 面板鉴定出 45 株（95.7%），MALDI-TOF MS 鉴定出 24 株（51.1%）（p <0.0000）。BCID2 检测到耐药基因 mecA/C (n = 16)、blaKPC (n = 8)；blaCTX-M (n = 17)、blaNDM (n = 8)、blaOXA-48 (n = 1) 和 vanA/B (n = 2)。BCID2 和 MALDI-TOF MS 报告结果的中位时间分别为 2.0 小时和 4.0 小时（p < 0.0000）。在分析的 124 个病例中，82.3%（102/124）的病例因 BCID2 的快速结果而改变了治疗方案。

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Optimización en la detección de bacteriemias. Uso integrado de FilmArray (BCID2) y MALDI-TOF

The usefulness of the combined use of MALDI-TOF MS from a subculture with 3-5 h of incubation and the BCID2 panel (FilmArray) for the identification of microorganisms from positive blood cultures and its importance in the adjustment of antimicrobial therapy was analyzed. Overall identification with BCID2 was 90.4% (142/157) and with Maldi-TOF MS 83.4% (131/157) (p = 0.0858); in 23 polymicrobial episodes (47 strains), the BCID2 panel identified 45 (95.7%) and MALDI-TOF MS 24 (51.1%) (p < 0.0000). BCID2 detected the presence of the resistance genes mecA/C (n = 16), bla_KPC (n = 8); bla_CTX-M (n = 17), bla_NDM (n = 8), bla_OXA-48 (n = 1), and vanA/B (n = 2). The median time to report a result was 2.0 h for BCID2 and 4.0 h for MALDI-TOF MS (p < 0.0000). Of 124 episodes analyzed, the rapid result of BCID2 led to 82.3% (102/124) therapeutic changes.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.