Natalia A. Carrion , Rodolfo E. Quiroz , Camila Asenzo , Manuela Gamarra , Antonio E. Flores , Maria Garcia , Rolando N. Soloaga , Julia Grigioni , Maria Mastroianni , Alejandra Margari , Facundo Molina , Adriana N. Procopio , Vanesa Reijtman , Maria S. Ratti , Victor Mamani , Myriam Vazquez , Diana Viale , Guadalupe Perez
{"title":"优化菌血症检测。综合使用 FilmArray (BCID2) 和 MALDI-TOF。","authors":"Natalia A. Carrion , Rodolfo E. Quiroz , Camila Asenzo , Manuela Gamarra , Antonio E. Flores , Maria Garcia , Rolando N. Soloaga , Julia Grigioni , Maria Mastroianni , Alejandra Margari , Facundo Molina , Adriana N. Procopio , Vanesa Reijtman , Maria S. Ratti , Victor Mamani , Myriam Vazquez , Diana Viale , Guadalupe Perez","doi":"10.1016/j.ram.2023.06.001","DOIUrl":null,"url":null,"abstract":"<div><p>The usefulness of the combined use of MALDI-TOF MS from a subculture with 3-5<!--> <!-->h of incubation and the BCID2 panel (FilmArray) for the identification of microorganisms from positive blood cultures and its importance in the adjustment of antimicrobial therapy was analyzed. Overall identification with BCID2 was 90.4% (142/157) and with Maldi-TOF MS 83.4% (131/157) (<em>p</em> <!-->=<!--> <!-->0.0858); in 23 polymicrobial episodes (47 strains), the BCID2 panel identified 45 (95.7%) and MALDI-TOF MS 24 (51.1%) (<em>p<!--> </em><<!--> <!-->0.0000). BCID2 detected the presence of the resistance genes <em>mec</em>A/C (n<!--> <!-->=<!--> <!-->16), <em>bla</em><sub>KPC</sub> (n<!--> <!-->=<!--> <!-->8); <em>bla</em><sub>CTX-M</sub> (n<!--> <!-->=<!--> <!-->17), <em>bla</em><sub>NDM</sub> (n<!--> <!-->=<!--> <!-->8), <em>bla</em><sub>OXA-48</sub> (n<!--> <!-->=<!--> <!-->1), and <em>van</em>A/B (n<!--> <!-->=<!--> <!-->2). The median time to report a result was 2.0<!--> <!-->h for BCID2 and 4.0<!--> <!-->h for MALDI-TOF MS (<em>p</em> <!--><<!--> <!-->0.0000). Of 124 episodes analyzed, the rapid result of BCID2 led to 82.3% (102/124) therapeutic changes.</p></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"55 4","pages":"Pages 332-336"},"PeriodicalIF":1.8000,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754123000524/pdfft?md5=9d1376e994dfb7eaf4494b58887faf4f&pid=1-s2.0-S0325754123000524-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Optimización en la detección de bacteriemias. Uso integrado de FilmArray (BCID2) y MALDI-TOF\",\"authors\":\"Natalia A. Carrion , Rodolfo E. Quiroz , Camila Asenzo , Manuela Gamarra , Antonio E. Flores , Maria Garcia , Rolando N. Soloaga , Julia Grigioni , Maria Mastroianni , Alejandra Margari , Facundo Molina , Adriana N. Procopio , Vanesa Reijtman , Maria S. Ratti , Victor Mamani , Myriam Vazquez , Diana Viale , Guadalupe Perez\",\"doi\":\"10.1016/j.ram.2023.06.001\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The usefulness of the combined use of MALDI-TOF MS from a subculture with 3-5<!--> <!-->h of incubation and the BCID2 panel (FilmArray) for the identification of microorganisms from positive blood cultures and its importance in the adjustment of antimicrobial therapy was analyzed. Overall identification with BCID2 was 90.4% (142/157) and with Maldi-TOF MS 83.4% (131/157) (<em>p</em> <!-->=<!--> <!-->0.0858); in 23 polymicrobial episodes (47 strains), the BCID2 panel identified 45 (95.7%) and MALDI-TOF MS 24 (51.1%) (<em>p<!--> </em><<!--> <!-->0.0000). BCID2 detected the presence of the resistance genes <em>mec</em>A/C (n<!--> <!-->=<!--> <!-->16), <em>bla</em><sub>KPC</sub> (n<!--> <!-->=<!--> <!-->8); <em>bla</em><sub>CTX-M</sub> (n<!--> <!-->=<!--> <!-->17), <em>bla</em><sub>NDM</sub> (n<!--> <!-->=<!--> <!-->8), <em>bla</em><sub>OXA-48</sub> (n<!--> <!-->=<!--> <!-->1), and <em>van</em>A/B (n<!--> <!-->=<!--> <!-->2). The median time to report a result was 2.0<!--> <!-->h for BCID2 and 4.0<!--> <!-->h for MALDI-TOF MS (<em>p</em> <!--><<!--> <!-->0.0000). 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Optimización en la detección de bacteriemias. Uso integrado de FilmArray (BCID2) y MALDI-TOF
The usefulness of the combined use of MALDI-TOF MS from a subculture with 3-5 h of incubation and the BCID2 panel (FilmArray) for the identification of microorganisms from positive blood cultures and its importance in the adjustment of antimicrobial therapy was analyzed. Overall identification with BCID2 was 90.4% (142/157) and with Maldi-TOF MS 83.4% (131/157) (p = 0.0858); in 23 polymicrobial episodes (47 strains), the BCID2 panel identified 45 (95.7%) and MALDI-TOF MS 24 (51.1%) (p < 0.0000). BCID2 detected the presence of the resistance genes mecA/C (n = 16), blaKPC (n = 8); blaCTX-M (n = 17), blaNDM (n = 8), blaOXA-48 (n = 1), and vanA/B (n = 2). The median time to report a result was 2.0 h for BCID2 and 4.0 h for MALDI-TOF MS (p < 0.0000). Of 124 episodes analyzed, the rapid result of BCID2 led to 82.3% (102/124) therapeutic changes.
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La Revista Argentina de Microbiología es una publicación trimestral editada por la Asociación Argentina de Microbiología y destinada a la difusión de trabajos científicos en las distintas áreas de la Microbiología. La Asociación Argentina de Microbiología se reserva los derechos de propiedad y reproducción del material aceptado y publicado.
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