完整三角蝽,1850(单属,辣椒科)的形态和分子特征,包括其完整的有丝分裂基因组。

IF 2.3 2区 医学 Q2 PARASITOLOGY
Parasite Pub Date : 2023-01-01 DOI:10.1051/parasite/2023016
Romain Gastineau, Chahinez Bouguerche, Fadila Tazerouti, Jean-Lou Justine
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引用次数: 1

摘要

辣椒素是在鱼的皮肤和鳃上发现的单子叶单系寄生虫。Capsalines (Capsalinae亚科)是一种大型的capsalides,寄生在非常珍贵的游戏鱼上,而Tristoma的物种只寄生在剑鱼(剑鱼)的鳃上。我们从地中海阿尔及利亚海域采集的旗鱼中获得了1850年的完整三棱鱼标本。在这里,我们描述了标本,包括背外侧体巩膜的主要系统学特征。一个标本被用于下一代测序分析,但它的一部分,包括硬结石,被安装在一个永久的载玻片上,绘制,并存放在一个精心策划的收藏中。我们描述了完整的有丝分裂基因组,核糖体簇(包括18S和28S)和额外的基因,如延伸因子1α (EF1α)和组蛋白3。我们还从单基因寄主组织中检索了存在于肠道中的分子信息,并提供了寄主X. gladius的完整rRNA簇的序列。其丝裂基因组长度为13 968 bp,编码12个蛋白、2个rRNA和22个tRNA。分别从28S序列和连接的线粒体蛋白编码基因进行了辣椒碱的系统发育。在28S系统发育中,大部分亚科的形态不属于单系,但辣椒科属于单系。在这两个系统发育中,最接近Tristoma sp .的成员是Capsaloides的成员。在附录中,我们报告了Tristoma Cuvier, 1817及其种的复杂命名史。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Morphological and molecular characterisation of Tristoma integrum Diesing, 1850 (Monogenea, Capsalidae), including its complete mitogenome.

Morphological and molecular characterisation of Tristoma integrum Diesing, 1850 (Monogenea, Capsalidae), including its complete mitogenome.

Morphological and molecular characterisation of Tristoma integrum Diesing, 1850 (Monogenea, Capsalidae), including its complete mitogenome.

Morphological and molecular characterisation of Tristoma integrum Diesing, 1850 (Monogenea, Capsalidae), including its complete mitogenome.

Capsalids are monopisthocotylean monogenean parasites found on the skin and gills of fish. Capsalines (subfamily Capsalinae) are large-sized capsalids, parasitic on highly prized gamefish, and species of Tristoma parasitise only the gills of swordfish (Xiphias gladius). We obtained specimens of Tristoma integrum Diesing, 1850 from swordfish collected off Algeria in the Mediterranean Sea. Here, we describe the specimens, including the key systematics characters of dorsolateral body sclerites. One specimen was used for a next generation sequencing analysis but a part of it, including the sclerites, was mounted on a permanent slide, drawn, and deposited in a curated collection. We characterised the complete mitogenome, the ribosomal cluster (including 18S and 28S) and additional genes such as Elongation factor 1 alpha (EF1α) and Histone 3. We also retrieved molecular information from the host tissue present in the gut of the monogenean and provide the sequence of the complete rRNA cluster of the host, X. gladius. The mitogenome of T. integrum is 13 968 bp in length and codes for 12 protein, 2 rRNA and 22 tRNA. Phylogenies of capsalids were generated from 28S sequences and concatenated mitochondrial protein-coding genes, respectively. In the 28S phylogeny, most subfamilies based on morphology were not found to be monophyletic, but the Capsalinae were monophyletic. In both phylogenies, the closest member to Tristoma spp. was a member of the Capsaloides. In an Appendix, we report the complex nomenclatural history of Tristoma Cuvier, 1817 and its species.

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来源期刊
Parasite
Parasite 医学-寄生虫学
CiteScore
5.50
自引率
6.90%
发文量
49
审稿时长
3 months
期刊介绍: Parasite is an international open-access, peer-reviewed, online journal publishing high quality papers on all aspects of human and animal parasitology. Reviews, articles and short notes may be submitted. Fields include, but are not limited to: general, medical and veterinary parasitology; morphology, including ultrastructure; parasite systematics, including entomology, acarology, helminthology and protistology, and molecular analyses; molecular biology and biochemistry; immunology of parasitic diseases; host-parasite relationships; ecology and life history of parasites; epidemiology; therapeutics; new diagnostic tools. All papers in Parasite are published in English. Manuscripts should have a broad interest and must not have been published or submitted elsewhere. No limit is imposed on the length of manuscripts, but they should be concisely written. Papers of limited interest such as case reports, epidemiological studies in punctual areas, isolated new geographical records, and systematic descriptions of single species will generally not be accepted, but might be considered if the authors succeed in demonstrating their interest.
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