CAPN3基因单核苷酸多态性与美利奴加鲁特回交羊生长性能的关系

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Puruhita, Ronny Rachman Noor, Endang Tri Margawati, Herman W Raadsma
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引用次数: 0

摘要

背景:绵羊是牲畜商品之一,在印度尼西亚被广泛认为是支持国家粮食安全的商品。通过选择生长遗传标记来提高遗传质量是提高肉类产量的必要条件。绵羊数量性状位点(QTL)分析表明,钙蛋白酶3基因(CAPN3)可能是影响绵羊生长性状的候选位点之一。CAPN3位于7号染色体上,在骨骼肌中表达。本研究旨在利用PCR-RLFP方法研究美利奴加鲁(MEGA)回交中CAPN3内含子11多态性及其与生长性状的相关性。结果:美利奴×加鲁特(MEGA)回交羊的SNP内含子11 CAPN3 | BseSI具有多态性,存在频率分别为0.76和0.24的C和T两个等位基因,以及频率分别为0.54、0.43和0.02的CC、CT和TT基因型。这些基因座处于Hardy-Weinberg平衡状态。结论:CAPN3 | BseSI可作为绵羊出生体重性状的遗传标记。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Association of the single nucleotide polymorphism in CAPN3 gene with growth performance in Merino and Garut (MEGA) backcross sheep.

Association of the single nucleotide polymorphism in CAPN3 gene with growth performance in Merino and Garut (MEGA) backcross sheep.

Association of the single nucleotide polymorphism in CAPN3 gene with growth performance in Merino and Garut (MEGA) backcross sheep.

Background: Sheep is one of the commodities of livestock which has been known widely in Indonesia for supporting the national food security. Improvement in genetic quality by selection based on genetic markers for growth is necessary to increase meat production. Quantitative trait loci (QTL) analysis in sheep suggests that Calpain 3 gene (CAPN3) gene might be one of the candidate loci affecting growth traits. CAPN3 is located on chromosome 7 sheep expressed in the skeletal muscles. The aim of this study was to investigate polymorphism CAPN3 intron 11 in Merino × Garut (MEGA) backcross using the PCR-RLFP method and to determine their association with growth traits.

Results: SNP intron 11 CAPN3 | BseSI of Merino × Garut (MEGA) backcross sheep was polymorphic and resulted in two alleles of C and T with a frequency of 0.76 and 0.24, respectively, and CC, CT, and TT genotypes with a frequency of 0.54, 0.43, and 0.02, respectively. These loci were found to be in Hardy-Weinberg equilibrium. The SNP CAPN3 | BseSI significantly affected (P < 0.05) the birth weight in Merino × Garut (MEGA) backcross sheep.

Conclusion: This result suggests that the CAPN3 | BseSI can be used as a genetic marker for birth weight trait in sheep.

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