纤维连接蛋白阳性的人“胶质样”细胞的星形胶质细胞分化。

IF 1.5 4区 医学 Q2 MEDICINE, GENERAL & INTERNAL
Ivana Sivakova, Peter Mraz, Anna Perzelova
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引用次数: 0

摘要

目的:纤维连接蛋白(Fn)是一种由多种间充质和肿瘤细胞类型产生的细胞外基质糖蛋白。背景:在成人脑组织中,Fn仅限于血管。然而,成人大脑培养物几乎完全由扁平或纺锤形的fn阳性细胞组成,通常被称为“神经胶质样”细胞。由于Fn主要存在于成纤维细胞中,这些培养物可能被认为是非神经胶质来源的。方法:采用免疫荧光法对12例非恶性诊断的成人脑组织进行细胞培养。结果:原代培养中含有GFAP-/Vim+/Fn+“胶质样”细胞(95% ~ 98%)和GFAP+/Vim+/Fn-星形胶质细胞(0.1%),传代3次后消失。12个培养体中有9个观察到细胞突的形成和细胞体的增大,细胞生长在第12至17代期间下降。值得注意的是,在此期间,所有“胶质样”细胞都变成了GFAP+/Vim+/Fn+。结论:在此,我们证实了我们之前发表的关于成人“胶质样”细胞起源的假设,我们认为这些细胞是分散在大脑皮层和皮层下白质中的前体细胞。培养物完全由GFAP-/Fn+“胶质样”细胞组成,并显示形态和免疫化学星形胶质细胞分化,随着传代时间的延长,生长自发减速。我们认为成人脑组织中含有一种未定义的神经胶质前体细胞的“休眠种群”。在培养下,这些细胞显示出高增殖能力和不同阶段的细胞去分化(图2,参考文献21)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Astroglial differentiation of fibronectin‑positive human "glia-like" cells.

Objectives: Fibronectin (Fn) is a glycoprotein of extracellular matrix produced by a variety of mesenchymal and neoplastic cell types.

Background: In adult brain tissue, Fn is restricted to blood vessels. However, adult human brain cultures are almost entirely comprised of flat or spindle‑shaped Fn-positive cells usually referred to as "glia-like" cells. Since Fn is primarily present in fibroblasts, these cultures may be considered to be of non-glial origin.

Methods: Cells gained by long-term culturing of adult human brain tissue derived from brain biopsies obtained from 12 patients with non-malignant diagnoses were examined by immunofluorescence methods.

Results: Primary cultures contained GFAP-/Vim+/Fn+ "glia-like" cells (95-98 %) and GFAP+/Vim+/Fn- astrocytes (0.1 %) which disappeared by passage number 3. The formation of cell processes and enlargement of cell bodies was observed in 9 of 12 cultures with decreased cell growth during passages 12 to 17. It is remarkable that during this period, all "glia-like" cells became GFAP+/Vim+/Fn+.

Conclusion: Herein, we confirm our previously published hypothesis about the origin of adult human "glia-like" cells, which we consider to be precursor cells scattered through the brain cortex and subcortical white matter. Cultures were comprised entirely of GFAP-/Fn+ "glia-like" cells and showed morphological and immunochemical astroglial differentiation with spontaneously decelerated growth during prolonged passaging. We propose that the adult human brain tissue contains a "dormant population" of undefined glial precursor cells. Under culture, these cells show to have a high proliferative capacity and different stages of cell dedifferentiation (Fig. 2, Ref. 21).

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来源期刊
CiteScore
2.60
自引率
0.00%
发文量
185
审稿时长
3-8 weeks
期刊介绍: The international biomedical journal - Bratislava Medical Journal – Bratislavske lekarske listy (Bratisl Lek Listy/Bratisl Med J) publishes peer-reviewed articles on all aspects of biomedical sciences, including experimental investigations with clear clinical relevance, original clinical studies and review articles.
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