壳聚糖衍生表观遗传调控支架在大鼠拔牙模型中保护牙槽嵴

IF 4.3 3区 材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC
ACS Applied Electronic Materials Pub Date : 2024-04-08 Epub Date: 2023-07-12 DOI:10.2186/jpr.JPR_D_23_00006
Teerawat Sukpaita, Suwabun Chirachanchai, Theerapat Chanamuangkon, Atiphan Pimkhaokham, Ruchanee Salingcarnboriboon Ampornaramveth
{"title":"壳聚糖衍生表观遗传调控支架在大鼠拔牙模型中保护牙槽嵴","authors":"Teerawat Sukpaita, Suwabun Chirachanchai, Theerapat Chanamuangkon, Atiphan Pimkhaokham, Ruchanee Salingcarnboriboon Ampornaramveth","doi":"10.2186/jpr.JPR_D_23_00006","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>Alveolar ridge preservation is a surgical technique used to prevent dimensional changes in the alveolar bone by dressing biomaterials in the extraction socket. Recently, a chitosan biphasic calcium phosphate loaded with trichostatin A (CS/BCP/TSA) scaffold was introduced as an excellent bone-regeneration material. This study aimed to explore the biological properties of released trichostatin A (TSA) and evaluate the potential of the CS/BCP/TSA scaffold in preserving the alveolar ridge in a rat tooth extraction model.</p><p><strong>Methods: </strong>In vitro biocompatibility, histone deacetylase (HDAC) activity, and osteogenic differentiation of MC3T3-E1 cells were tested. For in vivo studies, the maxillary first molars (M1) of Wistar rats were extracted, and alveolar ridge preservation was performed using a CS/BCP/TSA scaffold or commercial bone graft. Micro-Computed Tomography (micro-CT), polyfluorochrome labeling, and histological analysis were used to evaluate the ridge-preservation ability.</p><p><strong>Results: </strong>The released TSA was cytocompatible. Inhibition of histone deacetylase (HDAC) activity and induction of osteogenic differentiation in MC3T3-E1 cells were confirmed. The socket dressing with the CS/BCP/TSA scaffold showed increased socket bone fill and preserved the buccal and middle aspects of the alveolar ridge compared with the conventional graft. Further analysis of the bone regeneration ability by histomorphometric and histological analyses demonstrated that CS/BCP/TSA showed a significantly higher potential to induce bone formation and complete healing in the extraction socket than the other groups.</p><p><strong>Conclusions: </strong>The CS/BCP/TSA scaffold is a novel candidate for alveolar ridge preservation.</p>","PeriodicalId":3,"journal":{"name":"ACS Applied Electronic Materials","volume":null,"pages":null},"PeriodicalIF":4.3000,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Alveolar ridge preservation in rat tooth extraction model by chitosan-derived epigenetic modulation scaffold.\",\"authors\":\"Teerawat Sukpaita, Suwabun Chirachanchai, Theerapat Chanamuangkon, Atiphan Pimkhaokham, Ruchanee Salingcarnboriboon Ampornaramveth\",\"doi\":\"10.2186/jpr.JPR_D_23_00006\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Purpose: </strong>Alveolar ridge preservation is a surgical technique used to prevent dimensional changes in the alveolar bone by dressing biomaterials in the extraction socket. Recently, a chitosan biphasic calcium phosphate loaded with trichostatin A (CS/BCP/TSA) scaffold was introduced as an excellent bone-regeneration material. This study aimed to explore the biological properties of released trichostatin A (TSA) and evaluate the potential of the CS/BCP/TSA scaffold in preserving the alveolar ridge in a rat tooth extraction model.</p><p><strong>Methods: </strong>In vitro biocompatibility, histone deacetylase (HDAC) activity, and osteogenic differentiation of MC3T3-E1 cells were tested. For in vivo studies, the maxillary first molars (M1) of Wistar rats were extracted, and alveolar ridge preservation was performed using a CS/BCP/TSA scaffold or commercial bone graft. Micro-Computed Tomography (micro-CT), polyfluorochrome labeling, and histological analysis were used to evaluate the ridge-preservation ability.</p><p><strong>Results: </strong>The released TSA was cytocompatible. Inhibition of histone deacetylase (HDAC) activity and induction of osteogenic differentiation in MC3T3-E1 cells were confirmed. The socket dressing with the CS/BCP/TSA scaffold showed increased socket bone fill and preserved the buccal and middle aspects of the alveolar ridge compared with the conventional graft. Further analysis of the bone regeneration ability by histomorphometric and histological analyses demonstrated that CS/BCP/TSA showed a significantly higher potential to induce bone formation and complete healing in the extraction socket than the other groups.</p><p><strong>Conclusions: </strong>The CS/BCP/TSA scaffold is a novel candidate for alveolar ridge preservation.</p>\",\"PeriodicalId\":3,\"journal\":{\"name\":\"ACS Applied Electronic Materials\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.3000,\"publicationDate\":\"2024-04-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS Applied Electronic Materials\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.2186/jpr.JPR_D_23_00006\",\"RegionNum\":3,\"RegionCategory\":\"材料科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2023/7/12 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"ENGINEERING, ELECTRICAL & ELECTRONIC\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Electronic Materials","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.2186/jpr.JPR_D_23_00006","RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/7/12 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"ENGINEERING, ELECTRICAL & ELECTRONIC","Score":null,"Total":0}
引用次数: 0

摘要

目的:牙槽嵴保留是一种外科技术,通过在拔牙窝内敷生物材料来防止牙槽骨的尺寸变化。最近,壳聚糖双相磷酸钙负载三司他丁 A(CS/BCP/TSA)支架作为一种优良的骨再生材料被引入临床。本研究旨在探索释放的三氯他汀 A(TSA)的生物特性,并评估 CS/BCP/TSA 支架在大鼠拔牙模型中保存牙槽嵴的潜力:方法:测试体外生物相容性、组蛋白去乙酰化酶(HDAC)活性和 MC3T3-E1 细胞的成骨分化。在体内研究中,拔出 Wistar 大鼠的上颌第一臼齿(M1),使用 CS/BCP/TSA 支架或商业骨移植进行牙槽嵴保存。采用显微计算机断层扫描(micro-CT)、多氟铬标记和组织学分析来评估牙脊保存能力:结果:释放的 TSA 具有细胞相容性。结果:释放的 TSA 具有细胞相容性,可抑制组蛋白去乙酰化酶(HDAC)活性并诱导 MC3T3-E1 细胞成骨分化。使用 CS/BCP/TSA 支架包扎的牙槽敷料与传统移植材料相比,增加了牙槽骨的填充,并保留了牙槽嵴的颊侧和中部。通过组织形态学和组织学分析对骨再生能力的进一步分析表明,CS/BCP/TSA 在诱导骨形成和拔牙窝完全愈合方面的潜力明显高于其他组别:结论:CS/BCP/TSA 支架是牙槽嵴保存的一种新型候选材料。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Alveolar ridge preservation in rat tooth extraction model by chitosan-derived epigenetic modulation scaffold.

Purpose: Alveolar ridge preservation is a surgical technique used to prevent dimensional changes in the alveolar bone by dressing biomaterials in the extraction socket. Recently, a chitosan biphasic calcium phosphate loaded with trichostatin A (CS/BCP/TSA) scaffold was introduced as an excellent bone-regeneration material. This study aimed to explore the biological properties of released trichostatin A (TSA) and evaluate the potential of the CS/BCP/TSA scaffold in preserving the alveolar ridge in a rat tooth extraction model.

Methods: In vitro biocompatibility, histone deacetylase (HDAC) activity, and osteogenic differentiation of MC3T3-E1 cells were tested. For in vivo studies, the maxillary first molars (M1) of Wistar rats were extracted, and alveolar ridge preservation was performed using a CS/BCP/TSA scaffold or commercial bone graft. Micro-Computed Tomography (micro-CT), polyfluorochrome labeling, and histological analysis were used to evaluate the ridge-preservation ability.

Results: The released TSA was cytocompatible. Inhibition of histone deacetylase (HDAC) activity and induction of osteogenic differentiation in MC3T3-E1 cells were confirmed. The socket dressing with the CS/BCP/TSA scaffold showed increased socket bone fill and preserved the buccal and middle aspects of the alveolar ridge compared with the conventional graft. Further analysis of the bone regeneration ability by histomorphometric and histological analyses demonstrated that CS/BCP/TSA showed a significantly higher potential to induce bone formation and complete healing in the extraction socket than the other groups.

Conclusions: The CS/BCP/TSA scaffold is a novel candidate for alveolar ridge preservation.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
7.20
自引率
4.30%
发文量
567
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信