血清淀粉样蛋白A、血浆高密度脂蛋白胆固醇和载脂蛋白A- i的变化作为结核分枝杆菌感染的有用生物标志物

IF 2.4 4区医学 Q3 MICROBIOLOGY

Journal of medical microbiology Pub Date : 2023-06-01 DOI:10.1099/jmm.0.001726

Cleuber Franco Fontes, Nadielle Silva Bidu, Fatima Rodrigues Freitas, Raul Cavalcante Maranhão, Adriano de Souza Santos Monteiro, Ricardo David Couto, Eduardo Martins Netto

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Patients with TB symptoms attending the Instituto Brasileiro para a Investigação da Tuberculose/Fundação José Silveira (IBIT/FJS) between September 2015 and August 2016 for diagnosis of TB were studied. From 129 patients, 97 were classified as pulmonary TB and 32 as negative-bacilloscopy (non-TB group). Medical history, fasting serum and plasma were obtained. Total cholesterol (TC), HDL-C, apolipoprotein A-I and SAA were measured by enzymatic or immunochemical reaction assays. HDL size was measured by laser light-scattering.Results. In TB patients, TC (147.0±37 vs. 168±44 mg dL-1), HDL-C (37±14 vs. 55±18 mg dL-1) and apolipoprotein A-I (102±41 vs. 156±47 mg dL-1) concentrations were lower (P<0.0001), while HDL particle size (10.16±1.02 vs. 9.62±0.67 nm) and SAA levels (280±36 vs. 19±8 mg L-1) were higher (P<0.0001). 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引用次数: 0

摘要

介绍。近年来，由于有证据表明低血浆胆固醇水平与结核病(TB)之间存在关系，胆固醇受到了感染研究的关注。假设/差距语句。血清淀粉样蛋白A (SAA)、载脂蛋白A- i和高密度脂蛋白胆固醇(HDL-C)的血浆脂质谱是与症状性结核病患者相关的生物标志物。我们的目的是评估载脂蛋白A-I, SAA和高密度脂蛋白的大小作为诊断症状性结核病患者的生物标志物。研究了2015年9月至2016年8月期间在巴西结核病研究机构(IBIT/FJS)进行结核病诊断的有结核病症状的患者。129例患者中，97例为肺结核，32例为阴性杆菌镜检查(非结核组)。获得病史、空腹血清和血浆。采用酶法或免疫化学法测定总胆固醇(TC)、HDL-C、载脂蛋白A-I和SAA。用激光散射法测定高密度脂蛋白的大小。在结核病患者中，TC(147.0±37 vs。(168±44 mg dL-1)， HDL-C(37±14);(55±18 mg dL-1)和载脂蛋白A-I(102±41)。(156±47 mg dL-1)浓度较低(P-1)高于(P-1) (SAA的敏感性为96.88%，特异性为78.43%，HDL-C的敏感性为44.50 mg dL-1，敏感性为75%，特异性为72.16%，载脂蛋白A-I的敏感性为83.83%，特异性为72.22%，P118.5 mg dL-1)。SAA、HDL-C和载脂蛋白A-I与结核感染相关，可作为实验室生物标志物，特别是在抗酒精酸杆菌阴性的患者中。

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Changes in serum amyloid A, plasma high-density lipoprotein cholesterol and apolipoprotein A-I as useful biomarkers for Mycobacterium tuberculosis infection.

Introduction. In recent years, cholesterol has received interest in the study of infection due to evidence of a relationship between low plasma cholesterol levels and tuberculosis (TB).Hypothesis/Gap Statement. Plasma lipid profiles of serum amyloid A (SAA), apolipoprotein A-I and high-density lipoprotein cholesterol (HDL-C) are biomarkers associated with symptomatic TB patients.Objective. We aimed to evaluate plasma lipid profiles of apolipoprotein A-I, SAA and the size of HDL as biomarkers to diagnose symptomatic TB patients.Methodology. Patients with TB symptoms attending the Instituto Brasileiro para a Investigação da Tuberculose/Fundação José Silveira (IBIT/FJS) between September 2015 and August 2016 for diagnosis of TB were studied. From 129 patients, 97 were classified as pulmonary TB and 32 as negative-bacilloscopy (non-TB group). Medical history, fasting serum and plasma were obtained. Total cholesterol (TC), HDL-C, apolipoprotein A-I and SAA were measured by enzymatic or immunochemical reaction assays. HDL size was measured by laser light-scattering.Results. In TB patients, TC (147.0±37 vs. 168±44 mg dL^-1), HDL-C (37±14 vs. 55±18 mg dL^-1) and apolipoprotein A-I (102±41 vs. 156±47 mg dL^-1) concentrations were lower (P<0.0001), while HDL particle size (10.16±1.02 vs. 9.62±0.67 nm) and SAA levels (280±36 vs. 19±8 mg L^-1) were higher (P<0.0001). Using receiver-operating characteristic curve analysis for predicting TB, the cutoff values were <83.85 mg L^-1 for SAA (sensitivity=96.88 %, specificity=78.43 %, P<0.0001), >44.50 mg dL^-1 for HDL-C (sensitivity=75 %, specificity=72.16 %, P<0.001) and >118.5 mg dL^-1 for apolipoprotein A-I (sensitivity=83.83 %, specificity=72.22 %, P<0.001).Conclusion. SAA, HDL-C and apolipoprotein A-I are associated with TB infection and could be used as laboratory biomarkers, especially in patients who are negative for alcohol-acid-resistant bacilli.

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来源期刊

Journal of medical microbiology 医学-微生物学

CiteScore

5.50

自引率

3.30%

发文量

143

审稿时长

4.5 months

期刊介绍： Journal of Medical Microbiology provides comprehensive coverage of medical, dental and veterinary microbiology, and infectious diseases. We welcome everything from laboratory research to clinical trials, including bacteriology, virology, mycology and parasitology. We publish articles under the following subject categories: Antimicrobial resistance; Clinical microbiology; Disease, diagnosis and diagnostics; Medical mycology; Molecular and microbial epidemiology; Microbiome and microbial ecology in health; One Health; Pathogenesis, virulence and host response; Prevention, therapy and therapeutics