[微生物在法医鉴定中的实用价值(以历史墓葬遗骨的微生物菌群为例)]。

Q4 Medicine
N A Sidorova, O S Lavrukova, R F Beraya, V L Popov
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引用次数: 0

摘要

分析了从历史墓葬遗址遗骨表面分离的优势种的表型标志,以扩大遗骨微生物区系微生物多样性的数据,并评估微生物学分析结果在法医检验和法医考古证据基础中的应用可能性。在该历史遗址(年龄在90 ~ 95年之间)的骨碎片样品中,只有后菌科和真细菌定植于所有类型的表面;随着微菌的丰度增加,真菌的比例自然下降,而随着细菌背景计数的增加,微菌的检出率下降。骨残体中营养物质含量不高,导致污染骨残体的微生物数量和生物多样性下降;适应难以到达的有机基质的物种在那里占主导地位。在骨残体分解过程中,当其位置条件发生变化时,在给定的非生物和生物生存条件下,最适应难以到达的有机底物的微生物物种会发生种间竞争和特异性再定殖。所获得的结果对于描述死后微生物组中特定微生物组的生态学和生物学非常重要,并为更深入地研究骨残坏死微生物组中微生物物种之间的复杂交流奠定了基础-未来它将允许提出有关微生物参与物质和能量循环的原始假设。以及应用法医鉴定和法医考古证据库中获得的信息。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Practical value of microorganisms for forensic purposes (on the example of microbial flora of bony remnants from the historic burial site)].

Phenotypic signs of dominants isolated from the surface of bony remnants from the historic burial site were analyzed in order to expand data on the biodiversity of microorganisms in the microbial flora of bony remnants and to assess the possibility of using the results of microbiological analysis in the evidence base of forensic examination and forensic archaeology. It was detected that only Deuteromycota and Eubacteria colonized all types of surfaces in the samples of bone fragments from the historic burial site (with the age in the range of 90-95 years); with the abundance of micromycetes, the proportion of Eubacteria naturally decreased, while with the increased bacterial background counts the rate of micromycetes detection decreased. The insignificant amount of nutrients in the bony remnants led to the decrease in the number and biological diversity of microorganisms contaminating them; species adapted to a hard-to-reach organic substrate dominated there. During the process of bony remnants decomposition, when the conditions of their location changed, inter-species competition and specific recolonization occurred by species of microorganisms most adapted to a hard-to-reach organic substrate in the abiotic and biotic conditions of existence given. The results obtained are important for the descriptive ecology and biology of specific groups of microorganisms in the postmortem microbiome and form the basis for a more thorough study of complex communications between species of microorganisms in the necrobiome of bony remnants - in the future it will allow putting forward original hypotheses about the involvement of microbes in the circulation of matter and energy, as well as to apply the information obtained in the evidence base of forensic examination and forensic archaeology.

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来源期刊
Sudebno-Meditsinskaya Ekspertisa
Sudebno-Meditsinskaya Ekspertisa Medicine-Pathology and Forensic Medicine
CiteScore
0.60
自引率
0.00%
发文量
70
期刊介绍: The journal is concerned with the theory and practice of forensic medicine - the problems of thanatology, traumatology, toxicology, serology, forensic obstetrics, forensic dentistry, forensic psychiatry, forensic chemistry, physicotechnical methods of investigation, history of forensic medicine and some problems of criminology and legal laws related to forensic medicine. It publishes original studies by Russian authors, casuistry surveys, abstracts and reviews of Russian and foreign literature, scientific information, reports on scientific conferences.
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