在微生物诊断中应用二甲基硅氧烷防止培养基干燥。

IF 1.1 Q4 MEDICINE, RESEARCH & EXPERIMENTAL
Sovremennye Tehnologii v Medicine Pub Date : 2023-01-01 Epub Date: 2023-01-28 DOI:10.17691/stm2023.15.1.02
T A Savinova, Y A Bocharova, N A Mayansky, I V Chebotar
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引用次数: 0

摘要

寻找新的培养基改良方法以延长培养时间是微生物诊断进步的先决条件。本研究的目的是评估使用二甲基硅氧烷(聚甲基硅氧烷)作为琼脂表面与大气之间的屏障的可能性,以防止固体和半固体培养基的干燥,并保持其有用的特性:我们研究了微生物学所用培养基的水分(体积)损失动态,以及二甲基硅氧烷对这一过程的影响。二甲基硅氧烷在培养基表面分层排列。研究了二甲基硅氧烷对半固体琼脂中快速生长细菌(金黄色葡萄球菌、大肠杆菌、鼠伤寒沙门氏菌、伯克霍尔德氏伤寒杆菌)和慢速生长细菌(分枝杆菌)的生长和生成的影响,以及对细菌流动性(铜绿假单胞菌和大肠杆菌)的影响:结果:培养基中水分流失的动态变化表明,24 小时内所有不含二甲基硅氧烷的培养基(对照组)的重量损失均有统计学意义(金黄色假单胞菌、大肠杆菌、伤寒杆菌、塞氏杆菌),在不使用任何物质的对照组培养基和使用二甲基硅氧烷的培养基上没有显著差异。对照组中巧克力琼脂上可见的 M. avium 生长是在第 19 天,二甲基硅氧烷下是在第 18-19 天。在二甲基硅氧烷条件下,培养第 19 天的菌落数是对照组的十倍。24 小时后,铜绿假单胞菌和大肠杆菌在二甲基硅氧烷条件下半固体琼脂上的移动指数明显高于对照条件下的移动指数(p 结论:研究证实,在长时间培养的情况下,培养基的特性会明显恶化。所建议的使用二甲基硅氧烷保护培养基生长特性的技术显示出了良好的效果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Application of Dimethicone to Prevent Culture Media from Drying in Microbiological Diagnostics.

Application of Dimethicone to Prevent Culture Media from Drying in Microbiological Diagnostics.

The search for novel modifications of culture media aimed at culture prolongation is a prerequisite for microbiological diagnostic progress. The aim of the study was to assess the possibilities of applying dimethicone (polymethylsiloxane) as a barrier between the agar surface and atmosphere to prevent drying of solid and semisolid culture medium providing the retention of its useful properties.

Materials and methods: We studied the dynamics of water (volume) loss of culture media used in microbiology, and the effect of dimethicone on the process. Dimethicone was arranged in layers on culture medium surface. The effect of dimethicone on growth and generation of fast-growing (Staphylococcus aureus, Escherichia coli, Salmonella enterica Serovar Typhimurium, Burkholderia cenocepacia) and slow-growing (Mycobacterium avium) bacteria was studied, as well as on bacterial mobility (Pseudomonas aeruginosa and Escherichia coli) in semisolid agars.

Results: The dynamics of water loss in culture media showed the weight loss in all media without dimethicone (control) in 24 h to be statistically significant (p<0.05); 7-8 days later, they lost 50% of weight, and 14 days later they lost approximately 70%. The weight of media under dimethicone underwent no significant changes during the observation period. Growth index of fast-growing bacteria (S. aureus, E. coli, S. Typhimurium, B. cenocepacia) on control culture media without applying any substance, and on culture media under dimethicone had no significant differences. Visible M. avium growth on chocolate agar in controls was recorded on day 19, under dimethicone - on days 18-19. The number of colonies on culture day 19 under dimethicone tenfold exceeded the control values. The mobility indices of P. aeruginosa and E. coli on semisolid agar under dimethicone 24 h later were significantly higher than under control conditions (p<0.05 in both cases).

Conclusion: The study confirmed marked deterioration of culture media properties under prolonged cultivation. The suggested protection technology of culture media growth properties using dimethicone showed beneficial effects.

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来源期刊
Sovremennye Tehnologii v Medicine
Sovremennye Tehnologii v Medicine MEDICINE, RESEARCH & EXPERIMENTAL-
CiteScore
1.80
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38
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