原生质谱:最近的进展和仍然存在的挑战。

IF 10.4 1区生物学 Q1 BIOPHYSICS

Annual Review of Biophysics Pub Date : 2022-05-09 Epub Date: 2022-01-04 DOI:10.1146/annurev-biophys-092721-085421

Kelly R Karch, Dalton T Snyder, Sophie R Harvey, Vicki H Wysocki

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引用次数: 36

摘要

天然质谱法(nMS)已成为研究气相大分子及其配合物的结构和功能的重要工具。在这篇综述中，我们介绍了纳米粒子及其相关技术的最新进展，包括样品制备、仪器、激活方法和数据分析软件。这些进步使得基于纳米粒子的技术能够解决结构生物学中各种具有挑战性的问题。本综述的后半部分重点介绍了这些技术的最新应用，并调查了可以用nMS研究的配合物的类别。本文还讨论了纳米结构与现有结构生物学技术的互补性以及纳米结构面临的挑战。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Native Mass Spectrometry: Recent Progress and Remaining Challenges.

Native mass spectrometry (nMS) has emerged as an important tool in studying the structure and function of macromolecules and their complexes in the gas phase. In this review, we cover recent advances in nMS and related techniques including sample preparation, instrumentation, activation methods, and data analysis software. These advances have enabled nMS-based techniques to address a variety of challenging questions in structural biology. The second half of this review highlights recent applications of these technologies and surveys the classes of complexes that can be studied with nMS. Complementarity of nMS to existing structural biology techniques and current challenges in nMS are also addressed.

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来源期刊

Annual Review of Biophysics 生物-生物物理

CiteScore

21.00

自引率

0.00%

发文量

期刊介绍： The Annual Review of Biophysics, in publication since 1972, covers significant developments in the field of biophysics, including macromolecular structure, function and dynamics, theoretical and computational biophysics, molecular biophysics of the cell, physical systems biology, membrane biophysics, biotechnology, nanotechnology, and emerging techniques.