冷冻断裂电子显微镜揭示的膜脂亚细胞分布。

IF 1.2 4区医学 Q3 ANATOMY & MORPHOLOGY

Anatomical Science International Pub Date : 2023-06-14 DOI:10.1007/s12565-023-00731-9

Takuma Tsuji

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引用次数: 0

摘要

细胞膜由种类繁多的脂质和蛋白质组成。虽然人们已经对膜蛋白的定位和功能进行了广泛研究，但对膜脂质的分布，尤其是细胞器膜非细胞质小叶中的膜脂质的分布，仍然知之甚少。荧光生物传感器已被广泛用于研究膜脂的分布，但它们也有一些局限性。利用速冻和冻裂复制标记电子显微镜技术，我们可以揭示细胞内膜脂质的精确分布，并评估脂质运输蛋白的功能。在这篇综述中，我总结了利用这种方法分析细胞内脂质分布的最新进展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

$Subcellular distribution of membrane lipids revealed by freeze-fracture electron microscopy$

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Subcellular distribution of membrane lipids revealed by freeze-fracture electron microscopy

Cell membranes are composed of a large variety of lipids and proteins. While the localization and function of membrane proteins have been extensively investigated, the distribution of membrane lipids, especially in the non-cytoplasmic leaflet of organelle membranes, remains largely unknown. Fluorescent biosensors have been widely used to study membrane lipid distribution; however, they have some limitations. By utilizing the quick-freezing and freeze-fracture replica labeling electron microscopy technique, we can uncover the precise distribution of membrane lipids within cells and assess the function of lipid-transporting proteins. In this review, I summarize recent progress in analyzing intracellular lipid distribution by utilizing this method.

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来源期刊

Anatomical Science International 医学-解剖学与形态学

CiteScore

2.80

自引率

8.30%

发文量

审稿时长

>12 weeks

期刊介绍： The official English journal of the Japanese Association of Anatomists, Anatomical Science International (formerly titled Kaibogaku Zasshi) publishes original research articles dealing with morphological sciences. Coverage in the journal includes molecular, cellular, histological and gross anatomical studies on humans and on normal and experimental animals, as well as functional morphological, biochemical, physiological and behavioral studies if they include morphological analysis.