基于改良Sca/eS的小鼠耳蜗三维共聚焦显微镜成像

IF 1.2 4区医学 Q3 ANATOMY & MORPHOLOGY

Anatomical Science International Pub Date : 2023-02-11 DOI:10.1007/s12565-023-00703-z

Shinji Urata, Shigeo Okabe

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引用次数: 0

摘要

三维血管纹（SV）和耳蜗血管结构对内耳功能至关重要。本文报道了一种改良的Sca/eS，一种基于山梨醇的光学清除方法，用于显示完整小鼠耳蜗中的SV和血管结构。耳蜗巨噬细胞以及血管周围驻留的巨噬细胞样黑素细胞被检测为CX3CR1+/GFP小鼠的GFP阳性细胞。本研究的方法在生理和病理条件下都能有效地阐明内耳功能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Three-dimensional mouse cochlea imaging based on the modified Sca/eS using confocal microscopy

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Three-dimensional mouse cochlea imaging based on the modified Sca/eS using confocal microscopy

The three-dimensional stria vascularis (SV) and cochlear blood vessel structure is essential for inner ear function. Here, modified Sca/eS, a sorbitol-based optical-clearing method, was reported to visualize SV and vascular structure in the intact mouse cochlea. Cochlear macrophages as well as perivascular-resident macrophage-like melanocytes were detected as GFP-positive cells of the CX3CR1^+/GFP mice. This study’s method was effective in elucidating inner ear function under both physiological and pathological conditions.

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来源期刊

Anatomical Science International 医学-解剖学与形态学

CiteScore

2.80

自引率

8.30%

发文量

审稿时长

>12 weeks

期刊介绍： The official English journal of the Japanese Association of Anatomists, Anatomical Science International (formerly titled Kaibogaku Zasshi) publishes original research articles dealing with morphological sciences. Coverage in the journal includes molecular, cellular, histological and gross anatomical studies on humans and on normal and experimental animals, as well as functional morphological, biochemical, physiological and behavioral studies if they include morphological analysis.