A Ruiz Castillo, E López Herrero, A Tenorio Abreu, A González Gómez-Lozano, J M Saavedra Martín
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Gram staining of the right hand dorsum collection showed numerous polymorphonuclear leukocytes and no visible microorganisms. However, 4 days of incubation on blood agar at 35°C under 5% CO2 resulted in the formation of pinpoint-sized colonies resembling water droplets. These colonies could not be identified by matrix-assisted laser desorption ionization– time of flight mass spectrometry (MALDI-TOF MS) (“no peaks found”). Gram and Ziehl–Nielsen stainings performed on the colonies showed no bacteria. These results led us to suspect Mycoplasma spp, and the culture were transferred to Reference Laboratory for ARNr16s PCR and subsequent sequencing, where this microorganism was detected and identified.
期刊介绍:
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.