长链非编码RNA RP11-23J9.4在甲状腺癌中的作用及机制

IF 1.5 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Critical Reviews in Eukaryotic Gene Expression Pub Date : 2023-01-01 DOI:10.1615/CritRevEukaryotGeneExpr.2022046595

Lili Zhong, Xiangfu Ding, Xiaoliang Xiong, Tingting Hao, Chao Zhang, Lixing Wang, Yinlong Zhao

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引用次数: 0

摘要

简介:本研究旨在探讨LncRNA RP11 23J9.4是否可以作为治疗甲状腺癌(TC)的靶向标志物，LncRNA RP11 23J9.4下调与x射线辐射对TC有协同抑制作用。方法:通过细胞转染下调LncRNA RP11 23J9.4在甲状腺乳头状癌(PTC)细胞中的表达，并通过增殖、侵袭实验、细胞凋亡和细胞周期分析证实其对PTC细胞的抑制作用。转染后的细胞用2gy x射线照射。并采用上述方法检测其对TC是否具有协同抑制作用。采用实时荧光定量PCR、Western blotting、免疫组化检测Axin2基因及蛋白的表达。结果:一方面证明下调LncRNA RP11 23J9.4的表达可通过Axin2抑制TC的发生。另一方面，LncRNA RP11 23J9.4下调与x射线辐射对TC有协同抑制作用。结论:LncRNA RP11 23J9.4与x射线对TC有显著的协同作用。LncRNA RP11 23J9.4可作为TC靶向治疗的标志物。

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The Function and Mechanism of Long Non-Coding RNA RP11-23J9.4 in Thyroid Cancer.

Introduction: The objective of this research is to explore whether LncRNA RP11 23J9.4 can be used as a targeted marker for the treatment of thyroid cancer (TC), downregulation of LncRNA RP11 23J9.4 and X-ray radiation have synergistic inhibitory effect on TC.

Methods: The expression of LncRNA RP11 23J9.4 in papillary thyroid carcinoma (PTC) cell was downregulated by cell transfection, and its inhibitory effect on PTC cells was proved through proliferation, invasion experiment, apoptosis, and cell cycle analysis. The transfected cells were irradiated with 2 Gy X-ray. The above methods were also used to detect whether they had synergistic inhibitory effect on TC. The expression of Axin2 gene and protein were detected by real-time PCR, Western blotting, and immunohistochemistry.

Results: On the one hand, it is proved that downregulating the expression of LncRNA RP11 23J9.4 can inhibit the development of TC through Axin2. On the other hand, it is clear that downregulation of LncRNA RP11 23J9.4 and X-ray radiation have synergistic inhibitory effect on TC.

Conclusions: LncRNA RP11 23J9.4 and X-ray have significant synergistic effect on TC. LncRNA RP11 23J9.4 can be used as a marker for TC targeted therapy.

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来源期刊

Critical Reviews in Eukaryotic Gene Expression 生物-生物工程与应用微生物

CiteScore

2.70

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： Critical ReviewsTM in Eukaryotic Gene Expression presents timely concepts and experimental approaches that are contributing to rapid advances in our mechanistic understanding of gene regulation, organization, and structure within the contexts of biological control and the diagnosis/treatment of disease. The journal provides in-depth critical reviews, on well-defined topics of immediate interest, written by recognized specialists in the field. Extensive literature citations provide a comprehensive information resource. Reviews are developed from an historical perspective and suggest directions that can be anticipated. Strengths as well as limitations of methodologies and experimental strategies are considered.