联合使用细胞阻断和涂片提高细胞学诊断恶性肿瘤的不可触及的乳腺病变的影像学筛查。

IF 2.6 4区 医学 Q3 CELL BIOLOGY
Rieko Nishimura, Mikinao Oiwa
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引用次数: 0

摘要

背景:目前,芯针活检正在取代细针穿刺活检(FNAB)用于乳腺病变的病理诊断。然而,在我们医院,FNAB被广泛用于诊断乳腺病变,包括筛查的病变。此外,还使用了FNAB标本的直接涂片和细胞块(CBs)。为了制备CBs,常规使用苏木精和伊红(HE)染色以及p63和细胞角蛋白5/6抗体混合免疫染色。因此,在当前的研究中,我们试图评估使用常规涂片和CB免疫染色诊断乳腺病变的有效性。方法:对2014年12月至2020年3月来自名古屋医学中心的乳腺FNAB直接涂片和CBs报告进行回顾性分析。采用基于组织学的诊断方法比较直接涂片和CBs的诊断效率。结果:在169例组织学确诊的恶性病变中,直接涂片报告为不满意、良性或非典型可能良性的病变中,有12例经CB诊断为恶性。组织学上,这些病变被诊断为轻度异型或乳头状结构的癌。12个病变中有10个(83.3%)是不可触及的,只有在影像学上才能发现。结论:CB与常规涂片联合使用可在乳腺FNAB标本中检出更多的恶性病变,尤其是单纯影像学检出的病变。使用p63和细胞角蛋白5/6抗体混合免疫染色CB切片比单独HE染色提供更多信息。CB制备乳腺FNAB在发达国家已成功应用于乳腺病变评估。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Combined Use of Cell Block and Smear Improves the Cytological Diagnosis of Malignancy in Non-Palpable Breast Lesions Screened by Imaging.

Combined Use of Cell Block and Smear Improves the Cytological Diagnosis of Malignancy in Non-Palpable Breast Lesions Screened by Imaging.

Combined Use of Cell Block and Smear Improves the Cytological Diagnosis of Malignancy in Non-Palpable Breast Lesions Screened by Imaging.

Background: Currently, core needle biopsy is replacing fine needle aspiration biopsy (FNAB) for pathological diagnosis of breast lesions. However, FNAB is extensively used for diagnosing breast lesions, including screened lesions, at our hospital. Furthermore, direct smears as well as cell blocks (CBs) from the FNAB specimens have been used. To prepare the CBs, hematoxylin and eosin (HE) staining as well as immunostaining with a mixture of p63 and cytokeratin 5/6 antibodies are routinely used. Therefore, in the current study, we sought to assess the efficacy of diagnosing breast lesions using conventional smears and CB immunostaining.

Methods: Breast FNAB reports of direct smears and CBs from The Nagoya Medical Center between December 2014 and March 2020, were reviewed. The efficiency of diagnoses made with direct smears and CBs were compared using histology-based diagnoses.

Results: Among the 169 histologically confirmed malignant lesions, 12 lesions that were reported as unsatisfactory, benign, or atypia probably benign, using direct smears were diagnosed as malignant using CB. Histologically, these lesions were diagnosed as carcinomas with mild atypia or papillary structures. Ten (83.3%) of the twelve lesions were non-palpable and only detected upon imaging.

Conclusion: Combined use of CB and conventional smear leads to the detection of more malignant lesions in breast FNAB specimens, particularly in lesions detected by imaging alone. Immunostaining of CB sections using a mixture of p63 and cytokeratin 5/6 antibodies provides more information than HE staining alone. Breast FNAB with CB preparation can be successfully applied for evaluation of breast lesions in developed countries.

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来源期刊
Analytical Cellular Pathology
Analytical Cellular Pathology ONCOLOGY-CELL BIOLOGY
CiteScore
4.90
自引率
3.10%
发文量
70
审稿时长
16 weeks
期刊介绍: Analytical Cellular Pathology is a peer-reviewed, Open Access journal that provides a forum for scientists, medical practitioners and pathologists working in the area of cellular pathology. The journal publishes original research articles, review articles, and clinical studies related to cytology, carcinogenesis, cell receptors, biomarkers, diagnostic pathology, immunopathology, and hematology.
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