工程内溶素mtEC340M的晶体结构

IF 1.1 4区生物学 Q4 BIOCHEMICAL RESEARCH METHODS

Acta crystallographica. Section F, Structural biology communications Pub Date : 2023-05-03 DOI:10.1107/S2053230X23002583

Jee-Min Wang, Seung-Hyeon Seok, Won-Su Yoon, Ji-Hun Kim, Min-Duk Seo

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引用次数: 0

摘要

噬菌体产生的内溶素通过降解细菌细胞壁的肽聚糖层，在噬菌体后代的释放中发挥重要作用。噬菌体编码的内溶素已经成为一类新的抗菌剂，以对抗激增的抗生素耐药性。mtEC340M是一种从感染大肠杆菌的PBEC131噬菌体中提取的工程内溶素EC340，测定了其晶体结构。在2.4 Å分辨率下，mtEC340M的晶体结构由8个α-螺旋和2个环组成。通过与肽聚糖降解溶菌酶的结构比较，预测了mtEC340M的三个活性残基。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Crystal structure of the engineered endolysin mtEC340M

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Crystal structure of the engineered endolysin mtEC340M

Endolysins produced by bacteriophages play essential roles in the release of phage progeny by degrading the peptidoglycan layers of the bacterial cell wall. Bacteriophage-encoded endolysins have emerged as a new class of antibacterial agents to combat surging antibiotic resistance. The crystal structure of mtEC340M, an engineered endolysin EC340 from the PBEC131 phage that infects Escherichia coli, was determined. The crystal structure of mtEC340M at 2.4 Å resolution consists of eight α-helices and two loops. The three active residues of mtEC340M were predicted by structural comparison with peptidoglycan-degrading lysozyme.

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来源期刊

Acta crystallographica. Section F, Structural biology communications BIOCHEMICAL RESEARCH METHODSBIOCHEMISTRY &-BIOCHEMISTRY & MOLECULAR BIOLOGY

CiteScore

1.90

自引率

0.00%

发文量

期刊介绍： Acta Crystallographica Section F is a rapid structural biology communications journal. Articles on any aspect of structural biology, including structures determined using high-throughput methods or from iterative studies such as those used in the pharmaceutical industry, are welcomed by the journal. The journal offers the option of open access, and all communications benefit from unlimited free use of colour illustrations and no page charges. Authors are encouraged to submit multimedia content for publication with their articles. Acta Cryst. F has a dedicated online tool called publBio that is designed to make the preparation and submission of articles easier for authors.