环状RNA同源结构域相互作用蛋白激酶3和环状RNA 0046367通过Wnt/ β - catenin通路在埃及患者非酒精性脂肪性肝炎发病机制中的新作用

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Marwa Abdelgwad, Reem Zakaria, Samar Marzouk, Dina Sabry, Rasha Ahmed, Hedy Ayman Badary, Mai Samir
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引用次数: 0

摘要

背景:非酒精性脂肪性肝病是世界范围内的一个主要问题,需要非侵入性生物标志物进行早期诊断和治疗反应评估。我们旨在评估circRNA-HIPK3与miRNA-29a表达的相关性及其作为miRNA-29a海绵的作用,以及circRNA-0046367与miRNA-34a表达的相关性及其作为miRNA-34a海绵的作用及其调控Wnt/β catenin通路的作用,为非酒精性脂肪性肝炎的治疗提供新的靶点。方法:对110名参与者进行研究:第一组:55名健康供体作为对照;第二组:55名腹部超声显示脂肪肝的患者。评估血脂和肝功能。RT-PCR检测rna表达情况:circRNA-HIPK3、circRNA-0046367、miRNA-29a、miRNA-34a和Wnt mRNA基因表达情况。ELISA法检测β-catenin蛋白水平。结果:患者miRNA-34a和circRNA-HIPK3表达显著高于对照组,miRNA-29a和circRNA-0046367表达显著低于对照组。miRNA-29a和miRNA-34a调控的Wnt/β-catenin显著降低,导致其对脂质代谢的异常作用。结论:我们的研究结果表明,miRNA-29a可以作为circRNA-HIPK3的靶点,miRNA-34a可以作为circRNA-0046367的靶点,circRNA-HIPK3和circRNA-0046367可能具有通过Wnt/β-catenin通路影响非酒精性脂肪性肝炎发病机制的新作用,从而可作为该疾病的治疗靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Emerging Role of Circular RNA Homeodomain Interacting Protein Kinase 3 and Circular RNA 0046367 through Wnt/Beta-Catenin Pathway on the Pathogenesis of Nonalcoholic Steatohepatitis in Egyptian Patients.

Background: Non-alcoholic fatty liver disease is a major problem worldwide that needs non-invasive biomarkers for early diagnosis and treatment response assessment. We aimed to assess the correlation between circRNA-HIPK3 and miRNA-29a expression and its role as miRNA-29a sponge, as well as the correlation between circRNA-0046367 and miRNA-34a expression and its role as miRNA-34a sponge and their effect on regulation of the Wnt/β catenin pathway, which may provide a new target for treatment of non-alcoholic steatohepatitis.

Methods: the research was performed on 110 participants: group (I): fifty-five healthy donors served as controls and group (II): fifty-five patients with fatty liver pattern on abdominal ultrasound. Lipid profile and liver functions were assessed. RT-PCR was performed to assess the RNAs: circRNA-HIPK3, circRNA-0046367, miRNA-29a, miRNA-34a and Wnt mRNA gene expression. ELISA was performed to determine β-catenin protein levels.

Results: miRNA-34a and circRNA-HIPK3 expression were significantly greater, while miRNA-29a and circRNA-0046367 expression were significantly less, in patients than in controls. Wnt/β-catenin regulated by miRNA-29a and miRNA-34a showed a significant decrease that leads to its abnormal effect on lipid metabolism.

Conclusions: our results imply that miRNA-29a can be investigated as a target for circRNA-HIPK3, while miRNA-34a can be investigated as a target for circRNA-0046367, and that circRNA-HIPK3 and circRNA-0046367 may have emerging roles that can affect the pathogenesis of nonalcoholic steatohepatitis through the Wnt/β-catenin pathway and thus be used as therapeutic targets for the disease.

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来源期刊
Reports of Biochemistry and Molecular Biology
Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
2.80
自引率
23.50%
发文量
60
审稿时长
10 weeks
期刊介绍: The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
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