含有鸭天布苏病毒E蛋白结构域I和II的自组装纳米颗粒可以诱导更全面的免疫应答鸭天布苏病毒的攻击。

IF 1.3 4区 农林科学 Q2 VETERINARY SCIENCES
Qu Zhehui, Zhang Xiwen, Guo Xiaoqiu, Li Zhuoyan, Yu Wenjing, Lv Shuoshuo, Zhang Wen, Jiao Fengchao, He Shuhai, Lu Shaofang
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引用次数: 0

摘要

鸭坦布苏病毒(DTMUV)是一种致病性黄病毒,可导致家禽产蛋量大幅下降和严重的神经系统疾病。制备了具有E蛋白结构域I和II (edii -II- rfnp)的DTMUV自组装铁蛋白纳米颗粒(edii -II- rfnp),并对其形貌进行了观察。进行了两个独立的实验。首先,用EDI-II- rfnp、EDI-II和磷酸盐缓冲液(PBS, pH 7.4)接种14日龄樱桃谷鸭,血清中有特殊抗体和病毒中和抗体、白细胞介素4 (IL-4)和干扰素γ (IFN-γ),检测淋巴细胞增殖情况。其次,将接种了EDI-II- rfnp、EDI-II和PBS疫苗的鸭分别注射毒力强的DTMUV,观察感染后7天(dpi)的临床症状,并检测7和14 dpi时肺、肝和脑中DTMUV mRNA水平。结果表明,制备的EDI-II-RFNp纳米颗粒直径为16.46±4.70 nm,接近球形。EDI-II- rfnp组小鼠血清特异性抗体、VN抗体、IL-4和IFN-γ水平及淋巴细胞增殖均显著高于EDI-II和PBS组。在DTMUV攻击试验中,通过临床体征和组织mRNA水平来评估edii - ii - rfnp的保护作用。接种了edii - ii - rfnp的鸭子表现出较轻的临床症状,肺、肝和脑中的DTMUV RNA水平较低。这些结果表明,edii - ii - rfnp可以有效保护鸭子免受DTMUV的攻击,可以作为一种候选疫苗,为预防和控制DTMUV感染提供有效和安全的方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Self-Assembled Nanoparticles with E Protein Domains I and II of Duck Tembusu Virus Can Induce a More Comprehensive Immune Response Against the Duck Tembusu Virus Challenge.

Duck Tembusu virus (DTMUV) is a pathogenic flavivirus that causes a substantial drop in egg production and severe neurological disorders in domestic waterfowl. Self-assembled ferritin nanoparticles with E protein domains I and II (EDI-II) of DTMUV (EDI-II-RFNp) were prepared, and its morphology was observed. Two independent experiments were conducted. First, Cherry Valley ducks aged 14 days were vaccinated with EDI-II-RFNp, EDI-II, and phosphate buffered solution (PBS, pH 7.4), and special and virus neutralization (VN) antibodies, interleukin 4 (IL-4) and interferon gamma (IFN-γ) in serum, and lymphocyte proliferation were detected. Second, the vaccinated ducks with EDI-II-RFNp, EDI-II, and PBS were injected with virulent DTMUV, clinical signs at 7 days postinfection (dpi) were observed, and mRNA levels of DTMUV in the lungs, liver, and brain at 7 and 14 dpi were detected. The results showed near-spherical nanoparticles EDI-II-RFNp with a 16.46 ± 4.70 nm diameters. The levels of specific and VN antibodies, IL-4 and IFN-γ, and lymphocyte proliferation in the EDI-II-RFNp group were significantly higher than those in the EDI-II and PBS groups. In the DTMUV challenge test, clinical signs and mRNA levels in tissue were used to evaluate protection of EDI-II-RFNp. EDI-II-RFNp-vaccinated ducks showed milder clinical signs and lower levels of DTMUV RNA in the lungs, liver, and brain. These results indicate that EDI-II-RFNp effectively protects ducks against the DTMUV challenge and could be a vaccine candidate to provide an effective and safe method for preventing and controlling DTMUV infection.

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来源期刊
Avian Diseases
Avian Diseases 农林科学-兽医学
CiteScore
2.40
自引率
7.10%
发文量
80
审稿时长
3 months
期刊介绍: Avian Diseases is an international journal dedicated to publishing original basic or clinical research of the highest quality from various disciplines including microbiology, immunology, pathology and epidemiology. Papers on avian diseases relevant to etiology, pathogenesis, diagnosis, treatment, and control are accepted. Manuscripts dealing with avian species other than poultry will be considered only if the subject is relevant to poultry health.
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