{"title":"[低氧训练对骨骼肌中p53基因表达及其调控的线粒体有氧代谢信号通路的影响]。","authors":"Jie Li, Li-Li Zhao","doi":"10.12047/j.cjap.6314.2022.105","DOIUrl":null,"url":null,"abstract":"目的: 探讨低氧训练对大鼠骨骼肌p53及其调控的线粒体有氧代谢信号通路基因表达的影响,以及对线粒体有氧氧化供能能力的影响。方法: 30只雄性Wistar大鼠随机分为3组(n=10),低住低练组(LoLo)、高住高练组(HiHi)和高住高练低训组(HiHiLo)。以当地海拔1 500 m为常氧环境,模拟海拔3 500 m为低氧环境,各组大鼠按训练方案训练5周后,取股四头肌行匀浆及提取线粒体。Real-time PCR检测p53、细胞色素c氧化酶合成2(SCO2),细胞色素c氧化酶亚基Ⅰ(COXⅠ)和谷氨酰胺酶2(GLS2) mRNA表达,Westem blot检测 p53、SCO2、COXⅠ和GLS2蛋白表达;ELISA测定α-酮戊二酸脱氢酶(α-KGDHC),细胞色素c氧化酶(COX)及ATP合酶(ATP synthase)活性。结果: ①与LoLo 组比较,HiHi和HiHiLo组p53 mRNA水平显著升高(P＜ 0.01),HiHiLo组p53蛋白表达水平显著下降(P＜0.01);HiHi和HiHiLo组SCO2 mRNA水平和蛋白表达水平均显著升高(P＜ 0.01);HiHiLo组COXⅠmRNA水平显著升高(P＜0.01),HiHi组显著降低(P＜0.01),HiHi组COXⅠ蛋白表达水平显著升高(P＜ 0.05);HiHi和HiHiLo组GLS2 mRNA水平均显著升高(P＜0.05,P＜0.01),HiHiLo组GLS2蛋白表达水平显著下降(P＜0.01)。②与LoLo组比较,HiHi组ɑ-KGDHC和COX活性均显著降低(P＜0.01,P＜0.05),HiHiLo组均显著升高(P＜0.01);HiHi和HiHiLo组ATP synthase总活性和特异性活性均显著升高(P＜0.01)。结论: 高住高练低训上调大鼠骨骼肌p53及其调控的线粒体有氧代谢信号通路相关因子基因转录水平,提高骨骼肌线粒体有氧氧化供能能力。.","PeriodicalId":23985,"journal":{"name":"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Effects of hypoxic training on gene expressions of p53 and its regulated mitochondrial aerobic metabolic signaling pathway in skeletal muscle].\",\"authors\":\"Jie Li, Li-Li Zhao\",\"doi\":\"10.12047/j.cjap.6314.2022.105\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"目的: 探讨低氧训练对大鼠骨骼肌p53及其调控的线粒体有氧代谢信号通路基因表达的影响,以及对线粒体有氧氧化供能能力的影响。方法: 30只雄性Wistar大鼠随机分为3组(n=10),低住低练组(LoLo)、高住高练组(HiHi)和高住高练低训组(HiHiLo)。以当地海拔1 500 m为常氧环境,模拟海拔3 500 m为低氧环境,各组大鼠按训练方案训练5周后,取股四头肌行匀浆及提取线粒体。Real-time PCR检测p53、细胞色素c氧化酶合成2(SCO2),细胞色素c氧化酶亚基Ⅰ(COXⅠ)和谷氨酰胺酶2(GLS2) mRNA表达,Westem blot检测 p53、SCO2、COXⅠ和GLS2蛋白表达;ELISA测定α-酮戊二酸脱氢酶(α-KGDHC),细胞色素c氧化酶(COX)及ATP合酶(ATP synthase)活性。结果: ①与LoLo 组比较,HiHi和HiHiLo组p53 mRNA水平显著升高(P＜ 0.01),HiHiLo组p53蛋白表达水平显著下降(P＜0.01);HiHi和HiHiLo组SCO2 mRNA水平和蛋白表达水平均显著升高(P＜ 0.01);HiHiLo组COXⅠmRNA水平显著升高(P＜0.01),HiHi组显著降低(P＜0.01),HiHi组COXⅠ蛋白表达水平显著升高(P＜ 0.05);HiHi和HiHiLo组GLS2 mRNA水平均显著升高(P＜0.05,P＜0.01),HiHiLo组GLS2蛋白表达水平显著下降(P＜0.01)。②与LoLo组比较,HiHi组ɑ-KGDHC和COX活性均显著降低(P＜0.01,P＜0.05),HiHiLo组均显著升高(P＜0.01);HiHi和HiHiLo组ATP synthase总活性和特异性活性均显著升高(P＜0.01)。结论: 高住高练低训上调大鼠骨骼肌p53及其调控的线粒体有氧代谢信号通路相关因子基因转录水平,提高骨骼肌线粒体有氧氧化供能能力。.\",\"PeriodicalId\":23985,\"journal\":{\"name\":\"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.12047/j.cjap.6314.2022.105\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.12047/j.cjap.6314.2022.105","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 0
[Effects of hypoxic training on gene expressions of p53 and its regulated mitochondrial aerobic metabolic signaling pathway in skeletal muscle].
目的: 探讨低氧训练对大鼠骨骼肌p53及其调控的线粒体有氧代谢信号通路基因表达的影响,以及对线粒体有氧氧化供能能力的影响。方法: 30只雄性Wistar大鼠随机分为3组(n=10),低住低练组(LoLo)、高住高练组(HiHi)和高住高练低训组(HiHiLo)。以当地海拔1 500 m为常氧环境,模拟海拔3 500 m为低氧环境,各组大鼠按训练方案训练5周后,取股四头肌行匀浆及提取线粒体。Real-time PCR检测p53、细胞色素c氧化酶合成2(SCO2),细胞色素c氧化酶亚基Ⅰ(COXⅠ)和谷氨酰胺酶2(GLS2) mRNA表达,Westem blot检测 p53、SCO2、COXⅠ和GLS2蛋白表达;ELISA测定α-酮戊二酸脱氢酶(α-KGDHC),细胞色素c氧化酶(COX)及ATP合酶(ATP synthase)活性。结果: ①与LoLo 组比较,HiHi和HiHiLo组p53 mRNA水平显著升高(P＜ 0.01),HiHiLo组p53蛋白表达水平显著下降(P＜0.01);HiHi和HiHiLo组SCO2 mRNA水平和蛋白表达水平均显著升高(P＜ 0.01);HiHiLo组COXⅠmRNA水平显著升高(P＜0.01),HiHi组显著降低(P＜0.01),HiHi组COXⅠ蛋白表达水平显著升高(P＜ 0.05);HiHi和HiHiLo组GLS2 mRNA水平均显著升高(P＜0.05,P＜0.01),HiHiLo组GLS2蛋白表达水平显著下降(P＜0.01)。②与LoLo组比较,HiHi组ɑ-KGDHC和COX活性均显著降低(P＜0.01,P＜0.05),HiHiLo组均显著升高(P＜0.01);HiHi和HiHiLo组ATP synthase总活性和特异性活性均显著升高(P＜0.01)。结论: 高住高练低训上调大鼠骨骼肌p53及其调控的线粒体有氧代谢信号通路相关因子基因转录水平,提高骨骼肌线粒体有氧氧化供能能力。.
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