Jinying Luo, Yunfei Tang, Zhuannan Chu, Yuxin Peng, Jiawei Chen, Huiyang Yu, Chunmei Shi, Jahanzeb Jafar, Rong Chen, Yaping Tang, Yongen Lu, Zhibiao Ye, Ying Li, Bo Ouyang
{"title":"SlZF3通过直接抑制赤霉素合成途径中的SlGA20ox4调控番茄株高。","authors":"Jinying Luo, Yunfei Tang, Zhuannan Chu, Yuxin Peng, Jiawei Chen, Huiyang Yu, Chunmei Shi, Jahanzeb Jafar, Rong Chen, Yaping Tang, Yongen Lu, Zhibiao Ye, Ying Li, Bo Ouyang","doi":"10.1093/hr/uhad025","DOIUrl":null,"url":null,"abstract":"<p><p>Plant height is an important target trait for crop genetic improvement. Our previous work has identified a salt-tolerant C2H2 zinc finger, SlZF3, and its overexpression lines also showed a semi-dwarf phenotype, but the molecular mechanism remains to be elucidated. Here, we characterized the dwarf phenotype in detail. The dwarfism is caused by a decrease in stem internode cell elongation and deficiency of bioactive gibberellic acids (GAs), and can be rescued by exogenous GA3 treatment. Gene expression assays detected reduced expression of genes in the GA biosynthesis pathway of the overexpression lines, including <i>SlGA20ox4</i>. Several protein-DNA interaction methods confirmed that SlZF3 can directly bind to the <i>SlGA20ox4</i> promoter and inhibit its expression, and the interaction can also occur for <i>SlKS</i> and <i>SlKO</i>. Overexpression of <i>SlGA20ox4</i> in the <i>SlZF3</i>-overexpressing line can recover the dwarf phenotype. Therefore, SlZF3 regulates plant height by directly repressing genes in the tomato GA biosynthesis pathway.</p>","PeriodicalId":13179,"journal":{"name":"Horticulture Research","volume":"10 4","pages":"uhad025"},"PeriodicalIF":8.7000,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10116951/pdf/","citationCount":"1","resultStr":"{\"title\":\"SlZF3 regulates tomato plant height by directly repressing <i>SlGA20ox4</i> in the gibberellic acid biosynthesis pathway.\",\"authors\":\"Jinying Luo, Yunfei Tang, Zhuannan Chu, Yuxin Peng, Jiawei Chen, Huiyang Yu, Chunmei Shi, Jahanzeb Jafar, Rong Chen, Yaping Tang, Yongen Lu, Zhibiao Ye, Ying Li, Bo Ouyang\",\"doi\":\"10.1093/hr/uhad025\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Plant height is an important target trait for crop genetic improvement. Our previous work has identified a salt-tolerant C2H2 zinc finger, SlZF3, and its overexpression lines also showed a semi-dwarf phenotype, but the molecular mechanism remains to be elucidated. Here, we characterized the dwarf phenotype in detail. The dwarfism is caused by a decrease in stem internode cell elongation and deficiency of bioactive gibberellic acids (GAs), and can be rescued by exogenous GA3 treatment. Gene expression assays detected reduced expression of genes in the GA biosynthesis pathway of the overexpression lines, including <i>SlGA20ox4</i>. Several protein-DNA interaction methods confirmed that SlZF3 can directly bind to the <i>SlGA20ox4</i> promoter and inhibit its expression, and the interaction can also occur for <i>SlKS</i> and <i>SlKO</i>. Overexpression of <i>SlGA20ox4</i> in the <i>SlZF3</i>-overexpressing line can recover the dwarf phenotype. Therefore, SlZF3 regulates plant height by directly repressing genes in the tomato GA biosynthesis pathway.</p>\",\"PeriodicalId\":13179,\"journal\":{\"name\":\"Horticulture Research\",\"volume\":\"10 4\",\"pages\":\"uhad025\"},\"PeriodicalIF\":8.7000,\"publicationDate\":\"2023-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10116951/pdf/\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Horticulture Research\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1093/hr/uhad025\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Horticulture Research","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1093/hr/uhad025","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
SlZF3 regulates tomato plant height by directly repressing SlGA20ox4 in the gibberellic acid biosynthesis pathway.
Plant height is an important target trait for crop genetic improvement. Our previous work has identified a salt-tolerant C2H2 zinc finger, SlZF3, and its overexpression lines also showed a semi-dwarf phenotype, but the molecular mechanism remains to be elucidated. Here, we characterized the dwarf phenotype in detail. The dwarfism is caused by a decrease in stem internode cell elongation and deficiency of bioactive gibberellic acids (GAs), and can be rescued by exogenous GA3 treatment. Gene expression assays detected reduced expression of genes in the GA biosynthesis pathway of the overexpression lines, including SlGA20ox4. Several protein-DNA interaction methods confirmed that SlZF3 can directly bind to the SlGA20ox4 promoter and inhibit its expression, and the interaction can also occur for SlKS and SlKO. Overexpression of SlGA20ox4 in the SlZF3-overexpressing line can recover the dwarf phenotype. Therefore, SlZF3 regulates plant height by directly repressing genes in the tomato GA biosynthesis pathway.
期刊介绍：
Horticulture Research, an open access journal affiliated with Nanjing Agricultural University, has achieved the prestigious ranking of number one in the Horticulture category of the Journal Citation Reports ™ from Clarivate, 2022. As a leading publication in the field, the journal is dedicated to disseminating original research articles, comprehensive reviews, insightful perspectives, thought-provoking comments, and valuable correspondence articles and letters to the editor. Its scope encompasses all vital aspects of horticultural plants and disciplines, such as biotechnology, breeding, cellular and molecular biology, evolution, genetics, inter-species interactions, physiology, and the origination and domestication of crops.
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