人足月胎盘中SARS-CoV-2受体表达的免疫荧光研究

IF 2.9 4区 生物学 Q1 ANATOMY & MORPHOLOGY
Jürgen Becker, Danny Qiu, Walter Baron, Jörg Wilting
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引用次数: 0

摘要

直到2021年9月,严重急性呼吸综合征冠状病毒-2 (SARS-CoV-2;2019冠状病毒病(COVID-19)大流行造成2.17亿多人感染,450多万人死亡。在孕妇中,需要重症监护治疗的危险因素通常与总体人群相同。值得注意的是,covid -19阳性妇女比covid -19阴性妇女分娩早,新生儿和围产期严重发病率和死亡率的风险要高得多。病毒从孕妇向胎儿垂直传播的可能性和途径存在很大争议。最近的数据显示,在covid -19阳性妇女所生的416名新生儿中,有54名(13%)被感染。本研究以SARS-CoV-2大流行前收集的足月子胎盘为研究对象,研究了COVID-19主要受体血管紧张素转换酶2 (ACE2)、跨膜蛋白酶丝氨酸亚型2 (TMPRSS2)和神经泌素1 (NRP1)。我们对冷冻切片进行实时PCR和免疫荧光检测,并结合合胞滋养细胞、内皮细胞、巨噬细胞和基质细胞的标记。PCR研究显示,ACE2的截短型和长型都有表达。ACE2不与COVID-19刺突蛋白结合。所使用的ACE2抗体不能区分这两种形式。我们没有观察到典型的SARS-CoV-2进入机制在合胞细胞和细胞滋养细胞上的表达。ACE2和TMPRSS2在基质细胞亚群中共表达,其中部分是cd68阳性巨噬细胞。NRP1定位于内皮细胞。总而言之,胎盘一词并不是一个直接有利于COVID-19垂直传播的器官;然而,微创伤和胎盘炎可能削弱其屏障功能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Immunofluorescence Studies on the Expression of the SARS-CoV-2 Receptors in Human Term Placenta.

Immunofluorescence Studies on the Expression of the SARS-CoV-2 Receptors in Human Term Placenta.

Immunofluorescence Studies on the Expression of the SARS-CoV-2 Receptors in Human Term Placenta.

Immunofluorescence Studies on the Expression of the SARS-CoV-2 Receptors in Human Term Placenta.

Until September 2021, the Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2; COVID-19) pandemic caused over 217 million infections and over 4.5 million deaths. In pregnant women, the risk factors for the need of intensive care treatment are generally the same as in the overall population. Of note, COVID-19-positive women deliver earlier than COVID-19-negative women, and the risk for severe neonatal and perinatal morbidity and mortality is significantly higher. The probability and pathways of vertical transmission of the virus from the pregnant woman to the fetus are highly controversial. Recent data have shown that 54 (13%) of 416 neonates born to COVID-19-positive women were infected. Here, we investigated term placentas collected before the SARS-CoV-2 pandemic and studied the main COVID-19 receptors angiotensin-converting enzyme 2 (ACE2), transmembrane protease serine subtype 2 (TMPRSS2), as well as neuropilin 1 (NRP1). We performed real-time PCR and immunofluorescence on cryosections in combination with markers for syncytiotrophoblast, endothelial cells, macrophages and stromal cells. The PCR studies showed expression of both the truncated delta form of ACE2, which does not bind the COVID-19 spike protein, and the long form. The ACE2 antibody used does not distinguish between the two forms. We did not observe expression of the canonical SARS-CoV-2 entry machinery on syncytio- and cytotrophoblast. ACE2 and TMPRSS2 are co-expressed in a subpopulation of stromal cells, which in part are CD68-positive macrophages. NRP1 is localized to endothelial cells. In sum, the term placenta is not an organ that directly favors vertical transmission of COVID-19; however, microtraumas and placentitis may weaken its barrier function.

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来源期刊
Cells Tissues Organs
Cells Tissues Organs 生物-发育生物学
CiteScore
4.90
自引率
3.70%
发文量
45
审稿时长
6-12 weeks
期刊介绍: ''Cells Tissues Organs'' aims at bridging the gap between cell biology and developmental biology and the emerging fields of regenerative medicine (stem cell biology, tissue engineering, artificial organs, in vitro systems and transplantation biology). CTO offers a rapid and fair peer-review and exquisite reproduction quality. Special topic issues, entire issues of the journal devoted to a single research topic within the range of interests of the journal, are published at irregular intervals.
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