幼年雄性Dunkin-Hartley豚鼠(Cavia porcellus)头顶围栏监测的优化。

IF 1.3 4区 农林科学 Q2 VETERINARY SCIENCES
Laboratory Animals Pub Date : 2023-10-01 Epub Date: 2023-04-18 DOI:10.1177/00236772231165810
Joel E Helbling, Alexa P Spittler, Miranda J Sadar, Kelly S Santangelo
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引用次数: 0

摘要

头顶围栏监测为正在进行野外测试的动物提供了客观的定量移动性测量。值得注意的是,已经为豚鼠建立了最低限度的测试优化方案。尚不清楚(a)重复暴露、(b)一天中的时间或(c)测试持续时间的长短是否会影响结果参数。我们假设豚鼠在反复暴露于开阔场地后会表现出活动减少;在最早的测试期间活动增加;10 min将足以进行数据收集。这项研究分两个阶段进行,分别区分围栏习惯化和一天中的时间效应。两组雄性Dunkin-Hartley豚鼠被允许在开阔场地围栏内自愿活动14天 min来量化移动结果,包括移动的总距离、移动的总时间、移动时的平均速度和在避难所度过的总时间。对于这两个阶段,测试在一天中的四个不同时间进行,开销监控软件被编程为将总测试持续时间划分为2分钟。习惯化阶段的结果显示,重复暴露对时间流动和旅行距离有显著影响,因为动物在第一次测试中最活跃。在最早的测试期间,时间阶段的动物移动的时间明显更长。有趣的是,在一天中的时间阶段,在2分钟的垃圾箱中观察到显著差异,但在习惯化阶段没有。具体而言,随着测试持续时间的增加,观察到活动能力逐渐下降。因此,在可能的情况下,应该考虑习惯和一天中的时间。最后,试用期大于10 min可能不会产生额外的数据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Optimization of overhead enclosure monitoring in juvenile male Dunkin Hartley guinea pigs (Cavia porcellus).

Overhead enclosure monitoring provides objective quantitative mobility measurements for animals undergoing open-field testing. Notably, protocols for testing optimization have been minimally established for the guinea pig. It is unknown whether (a) repeated exposure, (b) time-of-day, or (c) length of testing duration influence outcome parameters. We hypothesized that guinea pigs would display decreased activity following repeated exposure to the open field; heightened activity during the earliest testing period; and that 10 min would be adequate for data collection. The study was conducted in two separate phases to distinguish between enclosure habituation and time-of-day effects, respectively. Two cohorts of male Dunkin Hartley guinea pigs were allowed voluntary movement in an open-field enclosure for 14 min to quantify mobility outcomes, including total distance traveled, total time mobile, average speed while mobile, and total time spent in the shelter. For both phases, testing occurred at four different times of day, and overhead monitoring software was programmed to divide the total testing duration into 2-min bins. Habituation phase results showed time mobile and distance traveled were influenced significantly by repeat exposure, as animals were most active during the first testing event. Time-of-day phase animals spent significantly more time mobile during the earliest testing period. Interestingly, significant differences were observed across 2-min bins for the time-of-day phase but not during the habituation phase. Specifically, progressively decreased ambulatory activity was observed as testing duration increased. Thus, habituation and time-of-day should be accounted for when possible. Finally, a trial period greater than 10 min may not yield additional data.

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来源期刊
Laboratory Animals
Laboratory Animals 生物-动物学
CiteScore
4.90
自引率
8.30%
发文量
64
审稿时长
6-12 weeks
期刊介绍: The international journal of laboratory animal science and welfare, Laboratory Animals publishes peer-reviewed original papers and reviews on all aspects of the use of animals in biomedical research. The journal promotes improvements in the welfare or well-being of the animals used, it particularly focuses on research that reduces the number of animals used or which replaces animal models with in vitro alternatives.
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