Saba Rana, Liaqat Hussain, Uzma Saleem, Muhammad Asif, Arslan H Lodhi, Muhammad Q Barkat, Muhammad Riaz, Alina Jamil
{"title":"黄藤水提物的剂量依赖性研究。来曲唑诱导雌性成年大鼠多囊卵巢综合征的作用机制探讨。","authors":"Saba Rana, Liaqat Hussain, Uzma Saleem, Muhammad Asif, Arslan H Lodhi, Muhammad Q Barkat, Muhammad Riaz, Alina Jamil","doi":"10.1177/15593258231169381","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Polycystic ovarian syndrome (PCOS) is an endocrine metabolic disorder of women.</p><p><strong>Purpose: </strong>This study aimed to explore the potential of aqueous extract of <i>Garcinia cambogia</i> Desr. (AEGC) in PCOS.</p><p><strong>Methodology: </strong>The HPLC was used to determine the phytoconstituents present in <i>Garcinia cambogia.</i> Thirty adult female albino rats were divided into 6 groups: Normal control (NC) disease Control (PCOS; letrozole 1 mg/kg), plant extract (AEGC 100, 300, 500 mg/kg) and standard (metformin; 20 mg/kg). Disease was confirmed by vaginal smear cytology. After 10 weeks, animals were euthanized, ovaries dissected for histopathology, blood collected for hormonal and biochemical analysis.</p><p><strong>Results: </strong>HPLC analysis showed the presence of phenolic contents; chlorogenic acid, gallic acid, coumaric acid while flavonoid contents were quercetin, kaempferol, and rutin. After treatment, there was dose dependent reduction of weight, ovarian cysts, improvement of follicle growth. DPPH radical scavenging percentage was 67.89%. Hormonal analysis showed a significant improvement (<i>P</i> < .05) in follicle stimulating hormone (FSH), estrogen, and progesterone while a reduction in testosterone, luteinizing hormone (LH) and insulin level. Antioxidant enzymatic markers were significantly (<i>P</i> < .05) increased. Lipid profile and LFTs were also improved.</p><p><strong>Conclusions: </strong>The study validated the potential of <i>Garcinia cambogia</i> in the management of PCOS.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":null,"pages":null},"PeriodicalIF":4.6000,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/90/c0/10.1177_15593258231169381.PMC10103256.pdf","citationCount":"3","resultStr":"{\"title\":\"Dose Dependent Effects of Aqueous Extract of <i>Garcinia cambogia</i> Desr. Against Letrozole Induced Polycystic Ovarian Syndrome in Female Adult Rats With Possible Mechanisms Exploration.\",\"authors\":\"Saba Rana, Liaqat Hussain, Uzma Saleem, Muhammad Asif, Arslan H Lodhi, Muhammad Q Barkat, Muhammad Riaz, Alina Jamil\",\"doi\":\"10.1177/15593258231169381\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Polycystic ovarian syndrome (PCOS) is an endocrine metabolic disorder of women.</p><p><strong>Purpose: </strong>This study aimed to explore the potential of aqueous extract of <i>Garcinia cambogia</i> Desr. (AEGC) in PCOS.</p><p><strong>Methodology: </strong>The HPLC was used to determine the phytoconstituents present in <i>Garcinia cambogia.</i> Thirty adult female albino rats were divided into 6 groups: Normal control (NC) disease Control (PCOS; letrozole 1 mg/kg), plant extract (AEGC 100, 300, 500 mg/kg) and standard (metformin; 20 mg/kg). Disease was confirmed by vaginal smear cytology. After 10 weeks, animals were euthanized, ovaries dissected for histopathology, blood collected for hormonal and biochemical analysis.</p><p><strong>Results: </strong>HPLC analysis showed the presence of phenolic contents; chlorogenic acid, gallic acid, coumaric acid while flavonoid contents were quercetin, kaempferol, and rutin. After treatment, there was dose dependent reduction of weight, ovarian cysts, improvement of follicle growth. DPPH radical scavenging percentage was 67.89%. Hormonal analysis showed a significant improvement (<i>P</i> < .05) in follicle stimulating hormone (FSH), estrogen, and progesterone while a reduction in testosterone, luteinizing hormone (LH) and insulin level. Antioxidant enzymatic markers were significantly (<i>P</i> < .05) increased. Lipid profile and LFTs were also improved.</p><p><strong>Conclusions: </strong>The study validated the potential of <i>Garcinia cambogia</i> in the management of PCOS.</p>\",\"PeriodicalId\":2,\"journal\":{\"name\":\"ACS Applied Bio Materials\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.6000,\"publicationDate\":\"2023-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/90/c0/10.1177_15593258231169381.PMC10103256.pdf\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS Applied Bio Materials\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1177/15593258231169381\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MATERIALS SCIENCE, BIOMATERIALS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Bio Materials","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1177/15593258231169381","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}
Dose Dependent Effects of Aqueous Extract of Garcinia cambogia Desr. Against Letrozole Induced Polycystic Ovarian Syndrome in Female Adult Rats With Possible Mechanisms Exploration.
Background: Polycystic ovarian syndrome (PCOS) is an endocrine metabolic disorder of women.
Purpose: This study aimed to explore the potential of aqueous extract of Garcinia cambogia Desr. (AEGC) in PCOS.
Methodology: The HPLC was used to determine the phytoconstituents present in Garcinia cambogia. Thirty adult female albino rats were divided into 6 groups: Normal control (NC) disease Control (PCOS; letrozole 1 mg/kg), plant extract (AEGC 100, 300, 500 mg/kg) and standard (metformin; 20 mg/kg). Disease was confirmed by vaginal smear cytology. After 10 weeks, animals were euthanized, ovaries dissected for histopathology, blood collected for hormonal and biochemical analysis.
Results: HPLC analysis showed the presence of phenolic contents; chlorogenic acid, gallic acid, coumaric acid while flavonoid contents were quercetin, kaempferol, and rutin. After treatment, there was dose dependent reduction of weight, ovarian cysts, improvement of follicle growth. DPPH radical scavenging percentage was 67.89%. Hormonal analysis showed a significant improvement (P < .05) in follicle stimulating hormone (FSH), estrogen, and progesterone while a reduction in testosterone, luteinizing hormone (LH) and insulin level. Antioxidant enzymatic markers were significantly (P < .05) increased. Lipid profile and LFTs were also improved.
Conclusions: The study validated the potential of Garcinia cambogia in the management of PCOS.
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