处理快速开花的迷你玉米

Q1 Agricultural and Biological Sciences

Current protocols in plant biology Pub Date : 2017-06-21 DOI:10.1002/cppb.20051

Morgan E. McCaw, James A. Birchler

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引用次数: 3

摘要

玉米(Zea mays)由于其易于杂交、后代数量相对较多以及能够通过表型核标记确定许多基因型性状而成为遗传分析的模式生物。快速开花迷你玉米A和B (FFMM)是最近开发的两个玉米品系，它们被选择为更有利于研究的性状。FFMM比标准玉米开花更快，而且小得多;因此，它需要一个稍微修改的协议，以照顾和交叉。以下方案描述了如何在温室、生长室和田间条件下种植、照料和杂交FFMM。©2017 by John Wiley &儿子,Inc。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Handling Fast-Flowering Mini-Maize

Maize (Zea mays) has a long history as a model organism for genetic analysis due to its ease of crossing, relatively large number of progeny, and ability to determine many genotypic traits through phenotypic kernel markers. Fast-Flowering Mini-Maize A and B (FFMM) are two recently developed lines of maize selected for traits that make them more conducive to research. FFMM flowers more quickly than standard maize and is much smaller; thus it requires a slightly modified protocol for care and crossing. The following protocol describes how to plant, care for, and cross FFMM in greenhouse, growth chamber, and field conditions. © 2017 by John Wiley & Sons, Inc.

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来源期刊

Current protocols in plant biology Agricultural and Biological Sciences-Plant Science

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期刊介绍： Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. Current Protocols in Plant Biology provides reproducible step-by-step instructions for protocols relevant to plant research. Furthermore, Current Protocols content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols in Plant Biology to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Plant Biology is the comprehensive source for protocols in the multidisciplinary field of plant biology, providing an extensive range of protocols from basic to cutting edge. Coverage includes: Extraction and analysis of DNA, RNA, proteins Chromosome analysis Transcriptional analysis Protein expression Metabolites Plant enzymology Epigenetics Plant genetic transformation Mutagenesis Arabidopsis, Maize, Poplar, Rice, and Soybean, and more.