聚焦突触:用于研究突触病的突触成像和质谱分析的最新进展。

IF 2.8 4区 医学 Q2 NEUROSCIENCES
Frontiers in Synaptic Neuroscience Pub Date : 2023-03-15 eCollection Date: 2023-01-01 DOI:10.3389/fnsyn.2023.1130198
Nicole Hindley, Anna Sanchez Avila, Christopher Henstridge
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引用次数: 0

摘要

突触是健康大脑功能不可或缺的组成部分,同时也越来越多地被认为是大脑疾病早期阶段的关键结构。了解驱动突触功能障碍的病理过程将为我们这个时代最具破坏性的一些疾病带来新的治疗机会。为此,我们需要一套可靠的成像和分子工具,以更高的分辨率来研究突触生物学。历史上,人们一直使用高技术成像模式对少量突触进行研究,或使用粗糙的分子方法对大量突触进行研究。然而,成像技术的最新进展使我们能够以单突触分辨率分析大量突触。此外,其中一些方法现在还可以实现多路复用,这意味着我们可以检查完整组织中单个突触的多种蛋白质。新的分子技术现在可以对分离的突触中的蛋白质进行精确定量。灵敏度越来越高的质谱分析设备的发展,意味着我们现在几乎可以全面扫描突触分子图谱,并观察其在疾病中的变化。随着这些新技术的发展,我们将能更清晰地观察突触,并在突触病领域获得更丰富、更有洞察力的高质量数据。在此,我们将以成像和质谱技术为重点,讨论方法学的进步如何促进对突触的研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Bringing synapses into focus: Recent advances in synaptic imaging and mass-spectrometry for studying synaptopathy.

Bringing synapses into focus: Recent advances in synaptic imaging and mass-spectrometry for studying synaptopathy.

Bringing synapses into focus: Recent advances in synaptic imaging and mass-spectrometry for studying synaptopathy.

Bringing synapses into focus: Recent advances in synaptic imaging and mass-spectrometry for studying synaptopathy.

Synapses are integral for healthy brain function and are becoming increasingly recognized as key structures in the early stages of brain disease. Understanding the pathological processes driving synaptic dysfunction will unlock new therapeutic opportunities for some of the most devastating diseases of our time. To achieve this we need a solid repertoire of imaging and molecular tools to interrogate synaptic biology at greater resolution. Synapses have historically been examined in small numbers, using highly technical imaging modalities, or in bulk, using crude molecular approaches. However, recent advances in imaging techniques are allowing us to analyze large numbers of synapses, at single-synapse resolution. Furthermore, multiplexing is now achievable with some of these approaches, meaning we can examine multiple proteins at individual synapses in intact tissue. New molecular techniques now allow accurate quantification of proteins from isolated synapses. The development of increasingly sensitive mass-spectrometry equipment means we can now scan the synaptic molecular landscape almost in totality and see how this changes in disease. As we embrace these new technical developments, synapses will be viewed with clearer focus, and the field of synaptopathy will become richer with insightful and high-quality data. Here, we will discuss some of the ways in which synaptic interrogation is being facilitated by methodological advances, focusing on imaging, and mass spectrometry.

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来源期刊
CiteScore
7.10
自引率
2.70%
发文量
74
审稿时长
14 weeks
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