光可控重组牛副流感病毒3型的产生

IF 1.9 4区医学 Q4 IMMUNOLOGY

Microbiology and Immunology Pub Date : 2023-01-06 DOI:10.1111/1348-0421.13052

Takashi Okura, Maino Tahara, Noriyuki Otsuki, Moritoshi Sato, Kaoru Takeuchi, Makoto Takeda

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引用次数: 0

摘要

牛副流感病毒3型(BPIV3)是一种很有前途的疫苗载体，可预防各种呼吸道病毒感染，包括人类PIV3、呼吸道合胞病毒和严重急性呼吸道综合征-冠状病毒2感染。在这项研究中，我们结合了磁铁系统和反向遗传方法来产生光可控的BPIV3。在BPIV3大蛋白基因的H2区插入一个光学可控的Magnet基因，该基因编码RNA依赖的RNA聚合酶。只有在蓝光照射下，生成的光可控BPIV3才能在细胞片的特定区域生长，这表明时空控制有助于BPIV3的安全临床应用。

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Generation of a photocontrollable recombinant bovine parainfluenza virus type 3

Bovine parainfluenza virus type 3 (BPIV3) is a promising vaccine vector against various respiratory virus infections, including the human PIV3, respiratory syncytial virus, and severe acute respiratory syndrome-coronavirus 2 infections. In this study, we combined the Magnet system and reverse genetic approach to generate photocontrollable BPIV3. An optically controllable Magnet gene was inserted into the H2 region of the BPIV3 large protein gene, which encodes an RNA-dependent RNA polymerase. The generated photocontrollable BPIV3 grew in specific regions of the cell sheet only when illuminated with blue light, suggesting that spatiotemporal control can aid in safe clinical applications of BPIV3.

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来源期刊

Microbiology and Immunology 医学-免疫学

CiteScore

5.20

自引率

3.80%

发文量

审稿时长

1 months

期刊介绍： Microbiology and Immunology is published in association with Japanese Society for Bacteriology, Japanese Society for Virology, and Japanese Society for Host Defense Research. It is peer-reviewed publication that provides insight into the study of microbes and the host immune, biological and physiological responses. Fields covered by Microbiology and Immunology include:Bacteriology|Virology|Immunology|pathogenic infections in human, animals and plants|pathogenicity and virulence factors such as microbial toxins and cell-surface components|factors involved in host defense, inflammation, development of vaccines|antimicrobial agents and drug resistance of microbes|genomics and proteomics.