新城疫/传染性支气管炎疫苗和饲用酵母制品对肉鸡前心窝和回肠先天免疫系统的影响

IF 1.8 4区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Yukinori Yoshimura, Takahiro Nii, Naoki Isobe
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引用次数: 0

摘要

本研究旨在探讨新城疫/传染性支气管炎(ND/IB)疫苗接种和酵母制品日粮添加是否能调节肉鸡前脑室和回肠先天免疫分子的表达。将1日龄雄性肉仔鸡分为V-Y-(对照组)、V-Y+、V+Y-和V+Y+组(V和Y分别代表接种和添加酵母制品)。V+Y-组和V+Y+组的雏鸡接种了ND/IB活疫苗,而V-Y-组和V-Y+组的雏鸡没有接种。V-Y+组和V+Y+组从第4天开始饲喂酵母制品饲料,V-Y-组和V+Y-组不饲喂酵母制品饲料。第7天采集胃前和回肠,分析7种toll样受体(TLRs)和Dectin-1的表达。在前脑室,与V+Y-对照组相比,V+Y-组的TLR7和TLR21表达水平较高;然而,在回肠中,任何TLR或Dectin-1的表达水平没有差异。在对照组和实验组之间,禽β-防御素和cathelicidin-1在前脑室和回肠的表达也没有差异。接种ND/IB疫苗后,细胞毒性细胞颗粒酶和白细胞介素-1B的表达上调。在雏鸡免疫ND/IB疫苗后,添加酵母制品只上调回肠颗粒酶的表达,下调前脑室IL-6的表达。因此,我们得出结论,至少在孵化后第7天之前,ND/IB疫苗接种对增强雏鸡前室先天免疫系统是有效的,而饲粮中添加酵母产品的效果可能有限,至少在目前的研究条件下是如此。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Effects of Newcastle Disease/Infectious Bronchitis Vaccine and Feeding Yeast Products on the Innate Immune System in the Proventriculus and Ileum of Broiler Chicks.

Effects of Newcastle Disease/Infectious Bronchitis Vaccine and Feeding Yeast Products on the Innate Immune System in the Proventriculus and Ileum of Broiler Chicks.

Effects of Newcastle Disease/Infectious Bronchitis Vaccine and Feeding Yeast Products on the Innate Immune System in the Proventriculus and Ileum of Broiler Chicks.

Effects of Newcastle Disease/Infectious Bronchitis Vaccine and Feeding Yeast Products on the Innate Immune System in the Proventriculus and Ileum of Broiler Chicks.

The aim of this study was to determine whether Newcastle disease/infectious bronchitis (ND/IB) vaccination and yeast product diet supplementation modulate the expression of innate immune molecules in the proventriculus and ileum of broiler chicks. One-day-old male broiler chicks were divided into four groups (V-Y- (control), V-Y+, V+Y-, and V+Y+ groups, where V and Y represent vaccination and yeast product supplementation, respectively). Chicks in the V+Y- and V+Y+ groups were immunized with the live ND/IB vaccine, whereas chicks in the V-Y- and V-Y+ groups were not. Chicks in the V-Y+ and V+Y+ groups received feed containing yeast products from day 4, whereas chicks in the V-Y- and V+Y- groups did not. The proventriculus and ileum were collected on day 7 to analyze the expression of seven Toll-like receptors (TLRs) and Dectin-1. In the proventriculus, compared with those of the V-Y- control group, the TLR7 and TLR21 expression levels were higher in the V+Y- group; however, there were no differences in the expression levels of any TLR or Dectin-1 in the ileum. There were also no differences in the expression of avian β-defensins and cathelicidin-1 in the proventriculus and ileum between the control and treatment groups. The expression of granzyme in cytotoxic cells and interleukin (IL)-1B was upregulated by ND/IB vaccination in the proventriculus. Supplementation with yeast products upregulated only granzyme expression in the ileum and downregulated IL-6 expression in the proventriculus in chicks immunized with the ND/IB vaccine. Thus, we concluded that ND/IB vaccination is effective at enhancing the innate immune system in the proventriculus of chicks, at least until day 7 post-hatching, whereas the effects of diet supplementation with yeast products may be limited, at least under the present study conditions.

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来源期刊
Journal of Poultry Science
Journal of Poultry Science AGRICULTURE, DAIRY & ANIMAL SCIENCE-
CiteScore
2.80
自引率
13.30%
发文量
26
审稿时长
12 months
期刊介绍: The Journal of Poultry Science will publish original reports and reviews which either make an original contribution to fundamental science or are of obvious application to the industry. Subjects which are covered include: breeding and genetics, nutrition and feeds, physiology, reproduction, immunology, behavior, environmental science, management and housing welfare, processing and products, and health in poultry. Submission of original articles to the Journal is open to all poultry researchers. The review articles are invited papers written by international outstanding researchers. Articles will be published in English, American style.
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