核因子-红细胞2相关因子2 (Nrf2)在围着床期小鼠子宫中的表达。

IF 1.6 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnic & Histochemistry Pub Date : 2023-02-01 DOI:10.1080/10520295.2022.2127156

Hakan Soylu, Kubra Aksu, Ezgi Golal, Ismail Ustunel, V Nimet Izgut-Uysal, Nuray Acar

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引用次数: 0

摘要

核因子-红细胞2相关因子- 2 (Nuclear factor-erythroid 2-related factor- 2, Nrf2)是一种促进解毒酶和抗氧化蛋白基因转录的核转录因子。我们观察Nrf2在围着床期的分布和表达。采用免疫组化、实时定量聚合酶链反应和western blotting检测小鼠发情期(对照组)和妊娠第1、4、5、6、8天子宫内Nrf2的表达。Nrf2免疫染色在妊娠第1、5、6天和妊娠第4、8天显著高于对照组;免疫印迹结果与免疫组化观察结果一致。第5、8天Nrf2 mRNA水平显著高于对照组。Nrf2在妊娠第1、5和6天的表达增加，可能通过保护发育中的胚胎和子宫免受氧化应激的不良影响，对子宫容受性、着床和脱胎化具有重要意义。

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Expression of nuclear factor-erythroid 2-related factor 2 (Nrf2) in mouse uterus during the peri-implantation period.

ABSTRACT Nuclear factor-erythroid 2-related factor- 2 (Nrf2) is a nuclear transcription factor that facilitates transcription of genes for detoxification enzymes and antioxidant proteins. We investigated the distribution and expression of Nrf2 during the peri-implantation period. We detected Nrf2 in uteri of mice during estrus (control) and on days 1, 4, 5, 6 and 8 of pregnancy using immunohistochemistry, quantitative real-time polymerase chain reaction and western blotting. Nrf2 immunostaining was significantly greater on days 1, 5 and 6 of pregnancy compared to controls, and on days 4 and 8 of pregnancy; western blotting results were consistent with immunohistochemical observations. Nrf2 mRNA levels on days 5 and 8 were significantly higher than for control uteri. Increased expression of Nrf2 on days 1, 5 and 6 of pregnancy may be important for uterine receptivity, implantation and decidualization by protecting the developing embryo and uterus from the adverse effects of oxidative stress.

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来源期刊

Biotechnic & Histochemistry 生物-生物工程与应用微生物

CiteScore

3.40

自引率

6.20%

发文量

审稿时长

6-12 weeks

期刊介绍： Biotechnic & Histochemistry (formerly Stain technology) is the official publication of the Biological Stain Commission. The journal has been in continuous publication since 1926. Biotechnic & Histochemistry is an interdisciplinary journal that embraces all aspects of techniques for visualizing biological processes and entities in cells, tissues and organisms; papers that describe experimental work that employs such investigative methods are appropriate for publication as well. Papers concerning topics as diverse as applications of histochemistry, immunohistochemistry, in situ hybridization, cytochemical probes, autoradiography, light and electron microscopy, tissue culture, in vivo and in vitro studies, image analysis, cytogenetics, automation or computerization of investigative procedures and other investigative approaches are appropriate for publication regardless of their length. Letters to the Editor and review articles concerning topics of special and current interest also are welcome.