基于Box-Behnken法的响应面建模优化群体淬灭介导的托鲁巴根提取物细菌衰减

IF 3.5 Q3 Biochemistry, Genetics and Molecular Biology
Kayeen Vadakkan , Selvaraj Vijayanand , Abbas Alam Choudhury , Ramya Gunasekaran , Janarthanam Hemapriya
{"title":"基于Box-Behnken法的响应面建模优化群体淬灭介导的托鲁巴根提取物细菌衰减","authors":"Kayeen Vadakkan ,&nbsp;Selvaraj Vijayanand ,&nbsp;Abbas Alam Choudhury ,&nbsp;Ramya Gunasekaran ,&nbsp;Janarthanam Hemapriya","doi":"10.1016/j.jgeb.2018.02.001","DOIUrl":null,"url":null,"abstract":"<div><p>The present study was intended to optimize the quorum sensing inhibitory action of <em>Solanum torvum</em> root extract against <em>Chromobacterium violaceum</em>. Factors such as bacterial density, frequency of administration and concentration of extract were analysed. Plant samples were collected from Thrissur District, Kerala, India. Response surface modelling of factors by Box-Behnken approach was employed for optimizing quorum quenching activity of extract. The adequacy of mathematical model was verified by ANOVA and Cook’s distance table. Results revealed that quorum quenching property of <em>Solanum torvum</em> root extract is highly influenced by variables studied whereas maximum activity was found during administration of 300 µg/ml extract thrice in a day. It was also understood that extract does not possess any bactericidal activity wherein it only silence its quorum sensing mediated functions. This observations can be further used in quorum quenching studies.</p></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":null,"pages":null},"PeriodicalIF":3.5000,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jgeb.2018.02.001","citationCount":"8","resultStr":"{\"title\":\"Optimization of quorum quenching mediated bacterial attenuation of Solanum torvum root extract by response surface modelling through Box-Behnken approach\",\"authors\":\"Kayeen Vadakkan ,&nbsp;Selvaraj Vijayanand ,&nbsp;Abbas Alam Choudhury ,&nbsp;Ramya Gunasekaran ,&nbsp;Janarthanam Hemapriya\",\"doi\":\"10.1016/j.jgeb.2018.02.001\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The present study was intended to optimize the quorum sensing inhibitory action of <em>Solanum torvum</em> root extract against <em>Chromobacterium violaceum</em>. Factors such as bacterial density, frequency of administration and concentration of extract were analysed. Plant samples were collected from Thrissur District, Kerala, India. Response surface modelling of factors by Box-Behnken approach was employed for optimizing quorum quenching activity of extract. The adequacy of mathematical model was verified by ANOVA and Cook’s distance table. Results revealed that quorum quenching property of <em>Solanum torvum</em> root extract is highly influenced by variables studied whereas maximum activity was found during administration of 300 µg/ml extract thrice in a day. It was also understood that extract does not possess any bactericidal activity wherein it only silence its quorum sensing mediated functions. This observations can be further used in quorum quenching studies.</p></div>\",\"PeriodicalId\":53463,\"journal\":{\"name\":\"Journal of Genetic Engineering and Biotechnology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.5000,\"publicationDate\":\"2018-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.jgeb.2018.02.001\",\"citationCount\":\"8\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Genetic Engineering and Biotechnology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1687157X18300076\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Biochemistry, Genetics and Molecular Biology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Genetic Engineering and Biotechnology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1687157X18300076","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 8

摘要

本研究旨在优化torvum根提取物对紫色杆菌的群体感应抑制作用。分析了细菌密度、给药频率和提取物浓度等因素。植物样本采集自印度喀拉拉邦Thrissur地区。采用Box-Behnken法对各因素进行响应面建模,优化提取物的群体猝灭活性。通过方差分析和库克距离表验证数学模型的充分性。结果表明,torvum根提取物的群体猝灭特性受研究变量的影响较大,其中300 µg/ml提取物每日3次时活性最大。还了解到,提取物不具有任何杀菌活性,其中它仅沉默其群体感应介导的功能。这一观察结果可以进一步用于群体猝灭研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Optimization of quorum quenching mediated bacterial attenuation of Solanum torvum root extract by response surface modelling through Box-Behnken approach

The present study was intended to optimize the quorum sensing inhibitory action of Solanum torvum root extract against Chromobacterium violaceum. Factors such as bacterial density, frequency of administration and concentration of extract were analysed. Plant samples were collected from Thrissur District, Kerala, India. Response surface modelling of factors by Box-Behnken approach was employed for optimizing quorum quenching activity of extract. The adequacy of mathematical model was verified by ANOVA and Cook’s distance table. Results revealed that quorum quenching property of Solanum torvum root extract is highly influenced by variables studied whereas maximum activity was found during administration of 300 µg/ml extract thrice in a day. It was also understood that extract does not possess any bactericidal activity wherein it only silence its quorum sensing mediated functions. This observations can be further used in quorum quenching studies.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Journal of Genetic Engineering and Biotechnology
Journal of Genetic Engineering and Biotechnology Biochemistry, Genetics and Molecular Biology-Biotechnology
CiteScore
5.70
自引率
5.70%
发文量
159
审稿时长
16 weeks
期刊介绍: Journal of genetic engineering and biotechnology is devoted to rapid publication of full-length research papers that leads to significant contribution in advancing knowledge in genetic engineering and biotechnology and provide novel perspectives in this research area. JGEB includes all major themes related to genetic engineering and recombinant DNA. The area of interest of JGEB includes but not restricted to: •Plant genetics •Animal genetics •Bacterial enzymes •Agricultural Biotechnology, •Biochemistry, •Biophysics, •Bioinformatics, •Environmental Biotechnology, •Industrial Biotechnology, •Microbial biotechnology, •Medical Biotechnology, •Bioenergy, Biosafety, •Biosecurity, •Bioethics, •GMOS, •Genomic, •Proteomic JGEB accepts
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信