LC-MS/MS快速测定小型猪血浆和牛奶中阿莫西林的方法:IMI ConcePTION项目贡献。

IF 1.3 4区 医学 Q4 PHARMACOLOGY & PHARMACY
Yeghig Armoudjian , Qi Lin , Bart Lammens , Johan Van Daele , Pieter Annaert
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引用次数: 0

摘要

IMI启动了“概念”项目,以填补在怀孕和哺乳期间使用药物的知识空白。为了实现这一目标,正在进行几项研究,包括对小型猪血浆和牛奶中的阿莫西林进行生物分析。根据FDA和EMA的指南,开发并验证了一种高通量、稳健可靠的液相色谱串联质谱法,用于测定大量小型猪血浆和牛奶样品中阿莫西林的浓度。色谱柱为Luna®Omega Polar C18, 1.6 μm, 100 × 2.1 mm,流动相为0.1%甲酸水溶液和乙腈。测定阿莫西林的质谱选择m/z 366.1→349.2,测定稳定同位素标记内标的质谱选择m/z 370.1→114.15。该方法线性定量范围为10 ng/mL ~ 10 μg/mL,回收率不小于94.1%,血浆和乳基质均采用单样品提取方法,分析运行时间为5 min。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Sensitive and rapid method for the quantitation of amoxicillin in minipig plasma and milk by LC-MS/MS: A contribution from the IMI ConcePTION project

The IMI project ConcePTION was launched to fill the knowledge gap of using medicines during pregnancy and lactation. To achieve this goal, several studies are being conducted, including the bioanalysis of amoxicillin in minipig plasma and milk. A high-throughput, robust and reliable liquid chromatography tandem mass spectrometry method was developed and validated according to FDA and EMA guidelines to determine the concentrations of amoxicillin in a large number of minipig plasma and milk samples. Chromatographic separation was achieved on a Luna® Omega Polar C18, 1.6 μm, 100 × 2.1 mm column, with a mobile phase consisting of 0.1% formic acid in water and acetonitrile. Mass spectrometry used in a positive ionization mode and the transitions m/z 366.1 → 349.2 was selected to monitor amoxicillin, while m/z 370.1 → 114.15 was selected for the stable isotope labelled internal standard. This method features a linear quantification range of 10 ng/mL - 10 μg/mL, recovery of not less than 94.1%, a single sample extraction method for both plasma and milk matrices, and an analysis runtime of 5 min.

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来源期刊
Journal of pharmacological and toxicological methods
Journal of pharmacological and toxicological methods PHARMACOLOGY & PHARMACY-TOXICOLOGY
CiteScore
3.60
自引率
10.50%
发文量
56
审稿时长
26 days
期刊介绍: Journal of Pharmacological and Toxicological Methods publishes original articles on current methods of investigation used in pharmacology and toxicology. Pharmacology and toxicology are defined in the broadest sense, referring to actions of drugs and chemicals on all living systems. With its international editorial board and noted contributors, Journal of Pharmacological and Toxicological Methods is the leading journal devoted exclusively to experimental procedures used by pharmacologists and toxicologists.
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