通过DNA修复推动下一代基因组编辑

IF 4.7 3区工程技术 Q2 ENGINEERING, BIOMEDICAL

Current Opinion in Biomedical Engineering Pub Date : 2023-09-22 DOI:10.1016/j.cobme.2023.100506

Ana Gvozdenovic, Jacob E. Corn

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引用次数: 0

摘要

基因组编辑技术产生靶向DNA损伤，并依赖于细胞DNA修复途径来解决。了解负责解决基因编辑工具引起的特定损伤的DNA修复机制可以显著推进它们的优化，并促进它们在研究和治疗环境中的更广泛应用。在这里，我们探讨了修复碱基和主要编辑器产生的DNA损伤的细胞过程，以及利用和操纵DNA修复途径以实现所需基因组变化的策略。

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Fueling next-generation genome editing with DNA repair

Genome editing technologies generate targeted DNA lesions and rely on cellular DNA repair pathways for resolution. Understanding the DNA repair mechanisms responsible for resolving the specific damage caused by gene editing tools can significantly advance their optimization and facilitate their broader application in research and therapeutic contexts. Here we explore the cellular processes involved in repairing base and prime editor-generated DNA lesions and strategies to leverage and manipulate DNA repair pathways for desired genomic changes.

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来源期刊

Current Opinion in Biomedical Engineering Medicine-Medicine (miscellaneous)

CiteScore

8.60

自引率

2.60%

发文量