泰国曼谷产青霉素酶淋病奈瑟菌分离株及其blatem135基因变异的分子流行病学研究

IF 1.3 4区 医学 Q4 INFECTIOUS DISEASES
Natakorn Nokchan, Perapon Nitayanon, Chanwit Tribuddharat
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引用次数: 0

摘要

具有blem -135的产青霉酶淋病奈瑟菌(PPNG)是严重的公共卫生威胁。只需要一个氨基酸序列的改变,blatemm -135就可以进化成tem型的广谱β -内酰胺酶(ESBL),它可以水解包括头孢曲松和头孢克肟在内的广谱头孢菌素。我们调查了PPNG分离株的分子流行病学特征、质粒类型以及产生TEM-135 β -内酰胺酶的PPNG临床分离株的流行情况。采用淋病奈瑟菌多抗原序列分型(NG-MAST)对2015 - 2017年采集的99株淋病奈瑟菌分离株进行分子流行病学特征分析。采用错配扩增突变法检测blatemm -135基因的流行情况。在鉴定的89个NG-MAST序列类型中,65个(73.0%)是新的。只有17.7%(43/243)的PPNG分离株属于16个基因群。最常见的质粒是非洲,其次是里约/多伦多和亚洲。在Rio/Toronto质粒中发现blem -135等位基因。23.2%(23/99)的PPNG分离株中存在blaem -135等位基因。表达TEM-135 β -内酰胺酶的PPNG分离株的青霉素最低抑制浓度(MIC)值显著高于TEM-1 PPNG分离株。PPNG分离株具有较高的遗传多样性和较高的blaem -135等位基因比例。blemam -135等位基因的突变令人担忧,因为只有一个突变可能导致TEM-1进化成一种降解头孢曲松的ESBL变体。对blaTEM-135和新的PPNG分离株进行持续监测是必要的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Molecular Epidemiology of Penicillinase-Producing Neisseria gonorrhoeae Isolates and Their blaTEM-135 Gene Variant in Bangkok, Thailand, 2015-2017.

Penicillinase-producing Neisseria gonorrhoeae (PPNG) possessing blaTEM-135 is a serious public health threat. With only a single change in the amino acid sequence, blaTEM-135 could evolve into a TEM-type extended-spectrum beta-lactamase (ESBL), which hydrolyzes extended-spectrum cephalosporins, including ceftriaxone and cefixime. We investigated the molecular epidemiological characteristics, types of plasmids in PPNG isolates, and prevalence of PPNG clinical isolates producing TEM-135 beta-lactamases. N. gonorrhoeae multi-antigen sequence typing (NG-MAST) was used to determine the molecular epidemiological characteristics of 99 PPNG isolates collected from 2015 to 2017. A mismatch amplification mutation assay was used to examine the blaTEM-135 gene prevalence. Of the 89 identified NG-MAST sequence types, 65 (73.0%) were novel. Only 17.7% (43/243) of PPNG isolates belonged to 16 genogroups. The most frequent plasmid was African, followed by Rio/Toronto, and Asian. The blaTEM-135 allele was found in Rio/Toronto plasmids. The blaTEM-135 allele was present in 23.2% (23/99) of the PPNG isolates. PPNG isolates expressing TEM-135 beta-lactamase exhibited significantly higher penicillin MIC (minimum inhibitory concentration) values than TEM-1 PPNG isolates. The PPNG isolates showed high genetic diversity and a high proportion of blaTEM-135 alleles. Mutation of the blaTEM-135 allele is worrisome as only one mutation could cause TEM-1 to evolve into an ESBL variant that degrades ceftriaxone. Ongoing surveillance of blaTEM-135 and new PPNG isolates is imperative.

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来源期刊
CiteScore
4.50
自引率
4.50%
发文量
172
审稿时长
2 months
期刊介绍: Japanese Journal of Infectious Diseases (JJID), an official bimonthly publication of National Institute of Infectious Diseases, Japan, publishes papers dealing with basic research on infectious diseases relevant to humans in the fields of bacteriology, virology, mycology, parasitology, medical entomology, vaccinology, and toxinology. Pathology, immunology, biochemistry, and blood safety related to microbial pathogens are among the fields covered. Sections include: original papers, short communications, epidemiological reports, methods, laboratory and epidemiology communications, letters to the editor, and reviews.
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