Emma L Keeler, Milica Vukmirovic, Xiting Yan, Kristin Gulino, Elodie Ghedin, Naftali Kaminski, Kathleen E Sullivan, Frederic D Bushman, Ronald G Collman, Misha Rosenbach
{"title":"结节病患者支气管肺泡灌洗细胞外病毒组和细胞转录组的宏基因组测序未检测到风疹病毒。","authors":"Emma L Keeler, Milica Vukmirovic, Xiting Yan, Kristin Gulino, Elodie Ghedin, Naftali Kaminski, Kathleen E Sullivan, Frederic D Bushman, Ronald G Collman, Misha Rosenbach","doi":"10.36141/svdld.v39i4.13407","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong> Sarcoidosis is a multisystem granulomatous inflammatory disease of unclear etiology that involves the lung, skin and other organs, with an unknown antigenic trigger. Recently, evidence has been found in both immune deficient and immune competent patients for rubella virus in cutaneous granulomas. These granulomatous lesions share overlapping features with cutaneous sarcoidosis, raising the question of rubella virus in sarcoidosis.</p><p><strong>Objective: </strong>To investigate the presence of rubella virus in sarcoidosis lung samples.</p><p><strong>Methods: </strong>We employed metagenomic sequencing to interrogate extracellular virome preparations and cellular transcriptomes from bronchoalveolar lavage (BAL) of 209 sarcoidosis patients for rubella virus sequences.</p><p><strong>Results: </strong>We found no evidence for rubella virus genomes in acellular fluid or rubella virus gene expression in BAL cells of sarcoidosis patients.</p><p><strong>Conclusions: </strong>These findings argue against rubella virus infection or persistence within the lung at time of sampling as a sarcoidosis trigger.</p>","PeriodicalId":21394,"journal":{"name":"Sarcoidosis, Vasculitis, and Diffuse Lung Diseases","volume":"39 4","pages":"e2022040"},"PeriodicalIF":1.4000,"publicationDate":"2022-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/5a/6a/SVDLD-39-40.PMC9798337.pdf","citationCount":"0","resultStr":"{\"title\":\"Metagenomic sequencing of the bronchoalveolar lavage extracellular virome and cellular transcriptome of sarcoidosis patients does not detect rubella virus.\",\"authors\":\"Emma L Keeler, Milica Vukmirovic, Xiting Yan, Kristin Gulino, Elodie Ghedin, Naftali Kaminski, Kathleen E Sullivan, Frederic D Bushman, Ronald G Collman, Misha Rosenbach\",\"doi\":\"10.36141/svdld.v39i4.13407\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong> Sarcoidosis is a multisystem granulomatous inflammatory disease of unclear etiology that involves the lung, skin and other organs, with an unknown antigenic trigger. Recently, evidence has been found in both immune deficient and immune competent patients for rubella virus in cutaneous granulomas. These granulomatous lesions share overlapping features with cutaneous sarcoidosis, raising the question of rubella virus in sarcoidosis.</p><p><strong>Objective: </strong>To investigate the presence of rubella virus in sarcoidosis lung samples.</p><p><strong>Methods: </strong>We employed metagenomic sequencing to interrogate extracellular virome preparations and cellular transcriptomes from bronchoalveolar lavage (BAL) of 209 sarcoidosis patients for rubella virus sequences.</p><p><strong>Results: </strong>We found no evidence for rubella virus genomes in acellular fluid or rubella virus gene expression in BAL cells of sarcoidosis patients.</p><p><strong>Conclusions: </strong>These findings argue against rubella virus infection or persistence within the lung at time of sampling as a sarcoidosis trigger.</p>\",\"PeriodicalId\":21394,\"journal\":{\"name\":\"Sarcoidosis, Vasculitis, and Diffuse Lung Diseases\",\"volume\":\"39 4\",\"pages\":\"e2022040\"},\"PeriodicalIF\":1.4000,\"publicationDate\":\"2022-12-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/5a/6a/SVDLD-39-40.PMC9798337.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Sarcoidosis, Vasculitis, and Diffuse Lung Diseases\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.36141/svdld.v39i4.13407\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"RESPIRATORY SYSTEM\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Sarcoidosis, Vasculitis, and Diffuse Lung Diseases","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.36141/svdld.v39i4.13407","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"RESPIRATORY SYSTEM","Score":null,"Total":0}
Metagenomic sequencing of the bronchoalveolar lavage extracellular virome and cellular transcriptome of sarcoidosis patients does not detect rubella virus.
Background: Sarcoidosis is a multisystem granulomatous inflammatory disease of unclear etiology that involves the lung, skin and other organs, with an unknown antigenic trigger. Recently, evidence has been found in both immune deficient and immune competent patients for rubella virus in cutaneous granulomas. These granulomatous lesions share overlapping features with cutaneous sarcoidosis, raising the question of rubella virus in sarcoidosis.
Objective: To investigate the presence of rubella virus in sarcoidosis lung samples.
Methods: We employed metagenomic sequencing to interrogate extracellular virome preparations and cellular transcriptomes from bronchoalveolar lavage (BAL) of 209 sarcoidosis patients for rubella virus sequences.
Results: We found no evidence for rubella virus genomes in acellular fluid or rubella virus gene expression in BAL cells of sarcoidosis patients.
Conclusions: These findings argue against rubella virus infection or persistence within the lung at time of sampling as a sarcoidosis trigger.
期刊介绍：
Sarcoidosis Vasculitis and Diffuse Lung Disease is a quarterly journal founded in 1984 by G. Rizzato. Now directed by R. Baughman (Cincinnati), P. Rottoli (Siena) and S. Tomassetti (Forlì), is the oldest and most prestigious Italian journal in such field.
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