Wiepko J. Terpstra , Geertruida S. Ligthart, Gerard J. Schoone
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Serodiagnosis of Human Leptospirosis by Enzyme-Linked-Immunosorbent-Assay (ELISA)
An enzyme-linked-immunosorbent-assay (ELISA) is described for the serodiagnosis of leptospirosis. Using an antigen prepared from a heated culture of a single leptospira strain (Wijnberg) the ELISA is a genusspecific test. The microscopic agglutination test (MAT) served as a reference. ELISA and MAT results agreed in 95% of the sera from 96 leptospirosis patients. One false positive was found in 217 controls. The ELISA is sensitive, specific and relatively easy to perform.
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