真核细胞的结构研究:从外围到内部的低温电子断层扫描

Q1 Biochemistry, Genetics and Molecular Biology

Advances in biological regulation Pub Date : 2023-01-01 DOI:10.1016/j.jbior.2022.100923

Chunyang Wang, Matthias Wojtynek, Ohad Medalia

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引用次数: 2

摘要

冷冻电子断层扫描（Cryo-ET）将细胞的近寿命保存与高分辨率三维（3D）成像相结合。这使得研究细胞景观的分子结构成为可能，并为生物过程和结构提供了前所未有的观点。在这篇综述中，我们主要关注冷冻ET在从外周到细胞内部的真核细胞可视化和结构表征中的应用。我们讨论了可用于研究具有挑战性的靶标在其细胞环境中的结构的策略，以及互补方法与冷冻-ET的应用。

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Structural investigation of eukaryotic cells: From the periphery to the interior by cryo-electron tomography

Cryo-electron tomography (cryo-ET) combines a close-to-life preservation of the cell with high-resolution three-dimensional (3D) imaging. This allows to study the molecular architecture of the cellular landscape and provides unprecedented views on biological processes and structures. In this review we mainly focus on the application of cryo-ET to visualize and structurally characterize eukaryotic cells – from the periphery to the cellular interior. We discuss strategies that can be employed to investigate the structure of challenging targets in their cellular environment as well as the application of complimentary approaches in conjunction with cryo-ET.

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来源期刊

Advances in biological regulation Biochemistry, Genetics and Molecular Biology-Molecular Medicine

CiteScore

8.90

自引率

0.00%

发文量

审稿时长

17 days