二氧化碳诱导下丘脑抗利尿激素神经元中c-Fos的表达

Prabha Kc , Musa A Haxhiu , C.Ovid Trouth , Kannan V Balan , Winston A Anderson , Serdia O Mack
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引用次数: 30

摘要

麻醉和未麻醉大鼠暴露于高碳水化合物应激后,检测下丘脑室旁核(PVN)和视上核(SON)含有精氨酸抗利尿素(AVP)的神经元c-fos基因编码蛋白(c-fos)的表达。此外,我们还测定了高碳酸血症激活的含avp的PVN神经元是否向膈核投射。在成年对照大鼠中,下丘脑核内仅观察到少量的c- fos样免疫反应神经元。c-Fos阳性细胞在呼吸含有高浓度CO2 (5% CO2, 21% O2和74% N2)的混合气体2小时后,或在呼吸12% CO2, 21% O2和67% N2后1小时后,被诱导显著增加。AVP和c-Fos蛋白的共定位研究显示,在PVN中,75%的AVP细胞表达c-Fos免疫反应性。c-Fos和AVP在麻醉大鼠60%的SON神经元中共表达。此外,用霍乱毒素b亚基(CTb)进行的逆行标记研究显示,如c-Fos表达所示,投射到膈核的PVN细胞亚群(15%)被高糖血症激活。这些结果表明(i) PVN和SON avp神经元是对高碳酸暴露作出反应的神经元网络的一部分;(ii)一部分CO2反应性PVN细胞支配膈核。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
CO2-induced c-Fos expression in hypothalamic vasopressin containing neurons

Following exposure of anesthetized and unanesthetized rats to hypercapnic stress, arginine vasopressin (AVP)-containing neurons of the hypothalamic paraventricular (PVN) and supraoptic (SON) nuclei were examined for expression of the c-fos gene encoded protein (c-Fos). In addition, we determined whether AVP-containing PVN neurons activated by hypercapnia project to phrenic nuclei. In adult control rats, only scant c-Fos-like immunoreactive neurons were observed within the hypothalamic nuclei. A marked increase in c-Fos positive cells was induced after 2 h of breathing a gas mixture with elevated CO2 (5% CO2, 21% O2 and 74% N2, or 1 h following breathing of 12% CO2, 21% O2, and 67% N2). Colocalization studies of AVP and c-Fos protein revealed that in the PVN, 75% of AVP-containing cells expressed c-Fos immunoreactivity. c-Fos and AVP were coexpressed in 60% of SON neurons in anesthetized rats. In addition, retrograde labeling studies with cholera toxin b subunit (CTb) revealed that a subpopulation of PVN cells (15%) that project to phrenic nuclei are activated by hypercapnia, as indicated by c-Fos expression. These results indicate that (i) PVN and SON AVP-containing neurons are part of the neuronal networks that react to hypercapnic exposure; and (ii) a subset of CO2 reactive PVN cells innervate phrenic nuclei.

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