Attempt to regenerate the dog’s tooth using the method of a new direction in biology‒Linguistic‒Wave Genetics

Multipotent mesenchymal stromal cells derived from human adipose tissue were used for transplantation. Cellular suspension from adipose tissue was diluted with Dulbecco’s Phosphate Buffered Saline (DPBS), (“Gibco”) 1:2, layered on a density gradient of Histopaque 1.077 (“Sigma”) and centrifuged for 30 minutes at 600g. Then, interfacial mononuclear rings were collected into centrifugal test tubes (“Corning”), washed by centrifugation in excess DPBS. The resulting cell sediment was resuspended in a culture medium and placed in culture test tubes (“Corning”, 25cm2) and transferred to a 37°C constant incubator with 5% carbon dioxide gas.