三叶草甲醇提取物的药理作用。(铁力科)在糖尿病肾病中的作用,特别提到抗氧化活性

Sarvesh Kumar Jatav
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摘要

目的:考察三叶草甲醇提取物的药理作用。在糖尿病肾病(DN),特别提到抗氧化活性。材料与方法:三叶草。采自当地高校校园药用园,经植物学家鉴定。依次用石油醚、二氯甲烷、乙酸乙酯、甲醇和水提取所选植物的干叶。在之前的研究中,甲醇提取物显示出了强大的活性,因此我们进一步选择了甲醇提取物作为其他模型。小鼠禁食过夜后腹腔单次注射新鲜制备的链脲佐菌素(50 mg/kg溶解于pH为4.5的柠檬酸缓冲液中),结扎左肾动脉诱导肾病。分别于第0、14、28天用眼反眶法采血,28天用眼测生化参数。取血后用离心机离心5000rpm 10min,采集血浆,自动分析仪测定不同生化参数。测定了丙二醛、谷胱甘肽、过氧化氢酶、超氧化物歧化酶等抗氧化指标。结果:第28天,DN组大鼠体重较正常对照组下降,提取物处理组大鼠体重较DN组显著升高。在第14、21、28天,DN组血糖水平较正常对照组明显升高,提取物处理组血糖水平较DN组明显降低。结论:本研究的总体结果清楚地表明,200和400 mg/kg剂量的提取物对糖尿病大鼠肾缺血再灌注肾病有改善作用。研究了三叶草甲醇提取物的肾保护作用、抗糖尿病作用和抗氧化作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of methanolic extract of Corchorus trilocularis Linn. (Tiliaceae) in diabetic nephropathy with special reference to antioxidant activity
Aim: Effect of methanolic extract of Corchorus trilocularis Linn. in diabetic nephropathy (DN) with special reference to anti-oxidant activity. Materials and Methods: C. trilocularis Linn. were collected from local medicinal garden of college campus and authentified by botanist. Dried leaves of selected plant were successively extracted using petroleum ether, dichloromethane, ethyl acetate, methanol, and finally water. In the previous study, methanolic extract showed potent activity so we have further selected methanolic extract for the other model. Animals were fasted for overnight then a single intra peritoneal injection of freshly prepared streptozotocin (50 mg/kg dissolved in citrate buffer pH 4.5) was injected and Nephropathy was induced by Ligation of the left renal artery. Blood was collected on 0, 14, and 28 day by retro-orbital on 28 days from eye for the estimation of biochemical parameters. The withdrawn blood was centrifuged at 5000 rpm for 10 min by using centrifuge and plasma was collected and different biochemical parameters were determined by auto analyzer. Some antioxidant parameters, that is, Malondialdehyde, glutathione, Catalase, and superoxide dismutase were also determined. Results: Here body weight of rat is decreased in DN group in comparison to normal control group while increased significantly in extract treated group at 28th day in comparison to DN group. Here blood glucose level of DN group was increased in comparison to normal control group while decreased significantly in extract treated group at 14th, 21st, and 28th days in comparison to DN group. Conclusion: The overall results of this study have clearly indicated that extract treated in a dose of 200 and 400 mg/kg ameliorate the renal ischemia reperfusion induced nephropathy in diabetic rats. The action of methanolic extract contributed by renoprotective, anti-diabetic, and antioxidant properties of C. trilocularis Linn.
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