印度尼西亚产eps7样肠杆菌噬菌体的鉴定

A. Atikana, K. Fujita, Alex Prima, Yopi Yopi, H. Kawasaki, Ken-ichiro Suzuki, P. Lisdiyanti
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引用次数: 0

摘要

噬菌体是地球上最丰富的实体。该目是细菌病毒中最大、分布最广的尾状病毒目。本研究的目的是表征来自印度尼西亚水域的噬菌体。在本次实验中,我们采集了印度尼西亚西爪哇的库宁安(Kuningan)和巴厘岛的Buleleng。以3株NBRC 13965、NBRC 12713和大肠杆菌NBRC 13168为宿主,采用覆盖法,结合PvuII、EcoRV和HincII三种限制性内切酶的酶切图谱和透射电子显微镜(TEM)对印尼产噬菌体的形态进行了表征。我们的研究结果表明,噬菌体LIPI13-Bp006与肠杆菌噬菌体EPS7属于一个高度相似的群体。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Characterization of EPS7-like Enterobacteria phage Isolated from Indonesia
Bacteriophages are the most abundant entities in earth. The order is the largest and most Caudovirales widespread group among bacterial viruses. The purpose of this study was to characterize bacteriophages from Indonesian waters. During this experiment, we collected sample from Kuningan (West Java) and Buleleng (Bali), Indonesia. We used an overlay agar method with three strains of as a host (NBRC 13965, NBRC 12713 and E. coli NBRC 13168) combined with digestion profiling using three restriction enzymes (PvuII, EcoRV and HincII) and transmission electron microscope (TEM) to characterize the morphology of the phage from Indonesia. Our results showed that phage LIPI13-Bp006 is in a group of and highly similar to Enterobacteria phage EPS7.
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