Magdalena Kolak, Joanna Gorecka, Malgorzata Radon-Pokracka, Maciej Piasecki, Agnieszka Cierniak, Agnieszka Micek, Anna Horbaczewska, Andrzej Jaworowski, Hubert Huras
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引用次数: 0
摘要
目的:许多物理和化学过程都会导致胎膜破裂。胎膜中存在多种金属蛋白酶,可导致 I 型胶原降解。I 型胶原的 C 端端肽(ICTP)是 I 型胶原的分解产物。该研究旨在确定在分娩前胎膜生理性破裂的情况下,ICTP是否会分泌到阴道宫颈液(VCF)中:研究于 2021 年 3 月在波兰克拉科夫的雅盖隆大学产科和围产医学系进行。研究共纳入 23 个病例。在使用窥器进行常规妇科检查时,收集两次 VCF,每次 50 微升。然后使用人 I 型胶原 C-三肽(ICTP)酶联免疫吸附试剂盒(目录编号:CSB-E10363h)对获得的材料进行酶联免疫测定。样品中 ICTP 的浓度已校准。该装置可检测的浓度范围为 25 ng /mL-800 ng/mL:结果:确认了 VCF 中存在 ICTP。最低浓度为 43.72 毫微克/毫升,最高浓度为 762.59 毫微克/毫升,有 5 个样品的浓度超出了仪器的最大检测范围:结论:ICTP 在孕妇生理分娩前的 VCF 中得到证实。需要进行进一步研究,以准确评估ICTP作为胎膜胶原降解过程的标志物在生理性分娩和胎膜早破机制中的作用。
ICTP concentration in cervical-vaginal fluid as a potential marker of membrane collagen degradation before labor.
Objectives: Numerous physical and chemical processes lead to rupture of membranes. Within the fetal membranes there are numerous types of metalloproteinases, which cause collagen type I degradation. The C-terminal telopeptide of colagen type I (ICTP) is the breakdown product of type I collagen. The aim of the study was to determine whether ICTP is secreted into the vaginal-cervical fluid (VCF) in the case of physiological rupture of the membranes of the fetus before delivery.
Material and methods: The study was conducted in March 2021 at the Department of Obstetrics and Perinatology of the Jagiellonian University in Cracow, Poland. Twenty-three cases were included in the study. During routine gynecological examination with the use of specula, VCF was collected twice in a volume of 50 µL. The obtained material was then subjected to enzyme immunoassay using the Human C-telopeptide of type I collagen (ICTP) ELISA Kit (Catalog Number. CSB-E10363h). The concentration of ICTP in the sample was calibrated. The concentration range that the device can detect was 25 ng /mL-800 ng/mL.
Results: The presence of ICTP in the VCF was confirmed. The minimum concentration was 43.72 ng/mL, the maximum was 762.59, in five cases the concentration was outside the maximum scale of the device.
Conclusions: ICTP was confirmed in the VCF of pregnant women before physiological delivery. Further studies are required to accurately evaluate ICTP as a marker of the processes of collagen degradation in fetal membranes in the mechanism of physiological labor and premature rupture of the membranes.
期刊介绍:
Ginekologia Polska’ is a monthly medical journal published in Polish and English language.
‘Ginekologia Polska’ will accept submissions relating to any aspect of gynaecology, obstetrics and areas directly related. ‘Ginekologia Polska’ publishes original contributions, comparative works, case studies, letters to the editor and many other categories of articles.