Effect of Nitrogen Sources and Illumination Conditions on Ganoderma lucidum Submerged Culture

The research was conducted to select the nitrogen sources and suitable light condition during Ganoderma lucidum submerged culture (isolated in Vietnam). The results showed that organic nitrogen is more suitable than inorganic nitrogen for the growth and synthesis of fungal polysaccharides. The amounts of incellular polysaccharide and exopolysaccharide in this strain was highest when combined 7.5 g/L yeast extract and 2.5 g/L peptone. The light affected on the size, shape and the color of the DL cells in submerged culture. Under optimum condition for nitrogen and light sources, the biomass, IPS and EPS content of DL strain were 13.90 ± 0.27 g DW/L, 15.33 ± 0.97 mg/100mg DW and 2349.76 ± 46.94 mg/L, respectively.