骨关节炎和糖尿病骨关节炎患者骨髓基质细胞体外释放细胞因子的研究。

Q3 Pharmacology, Toxicology and Pharmaceutics
Kar Wai Loh, Norshazliza Shaz, Simmrat Singh, Murali Malliga Raman, Hanumantha Rao Balaji Raghavendran, Tunku Kamarul
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引用次数: 2

摘要

目的:原发性骨关节炎(OA)是一种由特发性原因引起的进行性关节变性疾病。最近有证据表明OA与代谢综合征呈正相关。本初步研究旨在评估伴有或不伴有糖尿病(DM)的OA患者的促炎性和抗炎性细胞因子的基线水平,并评估过氧化氢(H2O2)对细胞因子产生的影响。方法:招募原发性髋关节和膝关节OA患者,在关节置换手术中采集3ml骨髓。分离骨髓基质细胞(Bone marrow stromal cells, BMSC),在培养瓶中培养3代。实验后在孔板中传代培养,标记为对照组和H2O2 (0.1 mM)处理组。采用ProcartaPlex®Multiplex Immunoassay测定0 h和72 h时BMSC产生的细胞因子水平。细胞因子,如肿瘤坏死因子- α、白细胞介素(IL)-6、IL-8和IL-1β,在糖尿病患者中普遍比非糖尿病患者在0和72小时时表现出更高的细胞因子水平。在0和72 h时,非糖尿病组和糖尿病组的表达相似。细胞因子IL-10在两组中没有显著差异,而DM和非糖尿病组在72 h时与对照组相比,IL-4水平下降。DM- oa的骨髓细胞更容易受到化学损伤,并且与较高水平的促炎细胞因子产生和较低水平的IL-4产生有关。结论:本研究提示骨关节炎合并糖尿病等合并症的治疗需要进一步研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cytokine release by human bone marrow stromal cells isolated from osteoarthritic and diabetic osteoarthritic patients in vitro.

Objectives: Primary Osteoarthritis (OA) is a disease of progressive joints degeneration due to idiopathic causes. Recent evidence showed a positive relationship between OA and metabolic syndrome. This pilot study aimed to assess the baseline level of pro and anti-inflammatory cytokines in OA patients with or without Diabetic Mellitus (DM) and assess the effect of hydrogen peroxide (H2O2) in cytokine production.

Methods: Patients with primary hip and knee OA were recruited, and 3 mL of bone marrow was harvested during joint replacement surgery. Bone marrow stromal cells (BMSC) was isolated and cultured in a culture flask for three passages. Later experiment was then sub-cultured in a well plate labeled as the control group and H2O2 (0.1 mM) treated group. ProcartaPlex® Multiplex Immunoassay was performed to measure cytokine levels produced by the BMSC at 0 h, as well as 72 h.

Results: Cytokines such as tumor necrosis factor-alpha, interleukin (IL)-6, IL-8, and IL-1β generally exhibited higher cytokine levels in subjects with DM than in nonDM subjects at 0 and 72 h. For IL-17, its expression was similar in nonDM and DM groups at 0 and 72 h. Cytokine IL-10 showed no significant difference in both the groups while DM and nonDM groups treated with H2O2 showed decreased IL-4 levels compared to control groups at 72 h. Bone marrow cells from DM-OA are more vulnerable to chemical insult and are associated with higher levels of proinflammatory cytokines production and lower IL-4 level production.

Conclusions: This study provides a clue that management of OA with co-morbidity like DM needs future studies.

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来源期刊
Journal of Basic and Clinical Physiology and Pharmacology
Journal of Basic and Clinical Physiology and Pharmacology Pharmacology, Toxicology and Pharmaceutics-Pharmacology
CiteScore
3.90
自引率
0.00%
发文量
53
期刊介绍: The Journal of Basic and Clinical Physiology and Pharmacology (JBCPP) is a peer-reviewed bi-monthly published journal in experimental medicine. JBCPP publishes novel research in the physiological and pharmacological sciences, including brain research; cardiovascular-pulmonary interactions; exercise; thermal control; haematology; immune response; inflammation; metabolism; oxidative stress; and phytotherapy. As the borders between physiology, pharmacology and biochemistry become increasingly blurred, we also welcome papers using cutting-edge techniques in cellular and/or molecular biology to link descriptive or behavioral studies with cellular and molecular mechanisms underlying the integrative processes. Topics: Behavior and Neuroprotection, Reproduction, Genotoxicity and Cytotoxicity, Vascular Conditions, Cardiovascular Function, Cardiovascular-Pulmonary Interactions, Oxidative Stress, Metabolism, Immune Response, Hematological Profile, Inflammation, Infection, Phytotherapy.
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